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© 2019

Progenitor Cells

Methods and Protocols

  • Mugdha V. Joglekar
  • Anandwardhan A. Hardikar

Benefits

  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts

Book

Part of the Methods in Molecular Biology book series (MIMB, volume 2029)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Sally Yunsun Kim, Thanh Huyen Phan, Christina Limantoro, Bill Kalionis, Wojciech Chrzanowski
    Pages 15-23
  3. Cody-Lee Maynard, Wilson K. M. Wong, Anandwardhan A. Hardikar, Mugdha V. Joglekar
    Pages 37-48
  4. Jessica Bruce, Gerard E. Kaiko, Simon Keely
    Pages 49-62
  5. Janine C. Quijano, Jacob R. Tremblay, Jeffrey Rawson, Hsun Teresa Ku
    Pages 63-80
  6. Arpita Poddar, Mugdha V. Joglekar, Anandwardhan A. Hardikar, Ravi Shukla
    Pages 81-91
  7. Luke Carroll, Auvro R. Mridha, Bernard E. Tuch
    Pages 93-102
  8. Badwi B. Boumelhem, Stuart T. Fraser, Stephen J. Assinder
    Pages 103-115
  9. Chanukya K. Colonne, Jia Hao Yeo, Campbell V. McKenzie, Stuart T. Fraser
    Pages 125-145
  10. Ashwini S. Hinge, Marie-Dominique Filippi
    Pages 147-160
  11. Parul Dixit, Rajesh G. Katare
    Pages 161-173
  12. Ayeshah A. Rosdah, Lea M. D. Delbridge, Shiang Y. Lim
    Pages 175-183
  13. Dario Gerace, Binhai Ren, Rosetta Martiniello-Wilks, Ann M. Simpson
    Pages 197-214
  14. Kisha N. Sivanathan, Patrick T. Coates
    Pages 215-234
  15. Sangeetha Vadakke-Madathil, Lalita S. Limaye, Vaijayanti P. Kale, Hina W. Chaudhry
    Pages 235-246
  16. Lawrence J. Liew, Allen Y. Wang, Rodney J. Dilley
    Pages 247-255

About this book

Introduction

This volume explores the diversity in progenitor cell biology methods and uniquely describes techniques for isolating, generating, and characterizing progenitor cells from either tissue or embryonic stem cells. The chapters in this book cover topics such as isolating progenitor cells from sources including adipose tissue, blood, bone marrow, ear, gut, heart, pancreatic islets, and Whirton’s jelly; deriving neural, pancreatic, and urothelial precursors from the embryonic stem cells; and culturing isolated islets in vitro to generate progenitor cells via epithelial-to-mesenchymal transition. This book also delves into digital droplet PCR; flow cytometry and cell sorting; mitochondrial assays; calcium ratiometric signaling; and 2D gel electrophoresis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Progenitor Cells: Methods and Protocols is a valuable resource for scientists and researchers interested in learning more about this evolving and diverse field. 

Keywords

Cell isolation Cellular differentiation Imaging techniques Cellular characterization methods Progenitor cell biology

Editors and affiliations

  • Mugdha V. Joglekar
    • 1
  • Anandwardhan A. Hardikar
    • 2
  1. 1.NHMRC Clinical Trials CentreFaculty of Medicine and Health, The University of SydneyCamperdownAustralia
  2. 2.NHMRC Clinical Trials CentreFaculty of Medicine and Health, The University of SydneyCamperdownAustralia

Bibliographic information

  • Book Title Progenitor Cells
  • Book Subtitle Methods and Protocols
  • Editors Mugdha V. Joglekar
    Anandwardhan A. Hardikar
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI https://doi.org/10.1007/978-1-4939-9631-5
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-9630-8
  • Softcover ISBN 978-1-4939-9633-9
  • eBook ISBN 978-1-4939-9631-5
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XIV, 289
  • Number of Illustrations 11 b/w illustrations, 57 illustrations in colour
  • Topics Stem Cells
  • Buy this book on publisher's site
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