© 2019


Methods and Protocols

  • Andreas Hiltbrunner
  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts


Part of the Methods in Molecular Biology book series (MIMB, volume 2026)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Eva Klement, Péter Gyula, András Viczián
    Pages 41-67
  3. Beatriz Orosa, András Viczián
    Pages 69-83
  4. Yun-Jeong Han, Jae-Yong Cho, Jeong-Il Kim
    Pages 95-111
  5. Cornelia Klose
    Pages 113-120
  6. Robert W. Smith, Christian Fleck
    Pages 121-133
  7. Chan Yul Yoo, Desiree Williams, Meng Chen
    Pages 135-141
  8. Tomonao Matsushita
    Pages 143-148
  9. Kijong Song, Giltsu Choi
    Pages 149-156
  10. Irma Roig-Villanova, Sandi Paulišić, Jaime F. Martinez-Garcia
    Pages 157-168
  11. Rachael J. Oakenfull, James Ronald, Seth J. Davis
    Pages 179-192
  12. Xu Wang, Qing Liu, Wenjin He, Chentao Lin, Qin Wang
    Pages 193-199
  13. Keisuke Inoue, Ryuichi Nishihama, Takayuki Kohchi
    Pages 215-223
  14. Anna Lena Ermert, Fabian Stahl, Tanja Gans, Jon Hughes
    Pages 225-236

About this book


This volume aims at providing readers with protocols to enhance their research for the next decade of phytochrome research. The chapters in this book cover topics such as tests for light-dependent interaction of phytochrome binding proteins using yeast two hybrid assays, in vitro pull down, and co-immunoprecipitation from stable transgenic lines; investigations into phosphorylation, SUMOylation, and ubiquitination of phytochromes and other proteins; a look at how in vivo spectroscopy can be used to measure Pfr levels of phytochrome in planta; identifying hormonal action and co-action in light-regulated processes; and ways to use the CRISPR/Cas9 system to generate Physcomitrella mutant lines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Phytochromes: Methods and Protocols serves as a guideline to researchers who are new to the field, and a stepping stone for experienced researchers looking to expand their knowledge of this evolving field. 


Co-IP assays Y2H assays ubiquitination chromophore mutants ChIP

Editors and affiliations

  • Andreas Hiltbrunner
    • 1
  1. 1.Faculty of Biology, Institute of Biology II and Signalling Research Centres BIOSS and CIBSSUniversity of FreiburgFreiburgGermany

Bibliographic information

  • Book Title Phytochromes
  • Book Subtitle Methods and Protocols
  • Editors Andreas Hiltbrunner
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-9611-7
  • Softcover ISBN 978-1-4939-9614-8
  • eBook ISBN 978-1-4939-9612-4
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XII, 278
  • Number of Illustrations 10 b/w illustrations, 30 illustrations in colour
  • Topics Plant Sciences
  • Buy this book on publisher's site
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