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Stem Cell Mobilization

Methods and Protocols

  • Gerd Klein
  • Patrick Wuchter
Book

Part of the Methods in Molecular Biology book series (MIMB, volume 2017)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Romy Kronstein-Wiedemann, Torsten Tonn
    Pages 29-40
  3. Julia Hümmer, Julian Koc, Axel Rosenhahn, Cornelia Lee-Thedieck
    Pages 71-84
  4. Eric Gottwald, Cordula Nies, Patrick Wuchter, Rainer Saffrich, Roman Truckenmüller, Stefan Giselbrecht
    Pages 85-95
  5. Sabrina Zippel, Annamarija Raic, Cornelia Lee-Thedieck
    Pages 97-107
  6. Jonas Stewen, Maria Gabriele Bixel
    Pages 109-121
  7. Ute Suessbier, César Nombela-Arrieta
    Pages 123-134
  8. Angela Jacobi, Philipp Rosendahl, Martin Kräter, Marta Urbanska, Maik Herbig, Jochen Guck
    Pages 135-148
  9. Andreas Maurer, Gerd Klein, Nicole D. Staudt
    Pages 149-163
  10. Petra Pavel, Sascha Laier
    Pages 177-192
  11. Martina Konantz, Joëlle S. Müller, Claudia Lengerke
    Pages 205-217
  12. Walter de Back, Thomas Zerjatke, Ingo Roeder
    Pages 219-243
  13. Back Matter
    Pages 245-248

About this book

Introduction

This book provides detailed state-of-the-art protocols for analyzing individual aspects of stem cell mobilization at the molecular and cellular level with contributions encompassing the most recent technological developments. Specifically, the volume covers cell adhesion and migration assays in three-dimensional models, imaging techniques for the determination of blood flow or hypoxic status, innovative biomechanical studies of stem cells, and assays to determine the involvement of proteases or complement factors, as well as other key topics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Stem Cell Mobilization: Methods and Protocols is an ideal guide for all laboratories in their endeavors to decipher the mechanisms of stem cell mobilization, ultimately helping stem cell donors and patients around the world.

Keywords

Stem cell harvesting Hematopoietic stem cells Transplantation Cell adhesion Migration assays

Editors and affiliations

  • Gerd Klein
    • 1
  • Patrick Wuchter
    • 2
  1. 1.Department of Internal Medicine IICenter for Medical Research, University of TübingenTübingenGermany
  2. 2.Institute of Transfusion Medicine and Immunology, Medical Faculty MannheimHeidelberg UniversityMannheimGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-9574-5
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9573-8
  • Online ISBN 978-1-4939-9574-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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