© 2019

Microbial Transposon Mutagenesis

Protocols and Applications

  • Steven C. Ricke
  • Si Hong Park
  • Morgan L. Davis

Part of the Methods in Molecular Biology book series (MIMB, volume 2016)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Transposon Protocols for Specific Microorganisms

    1. Front Matter
      Pages 1-1
    2. Sardar Karash, Tieshan Jiang, Deepti Samarth, Reena Chandrashekar, Young Min Kwon
      Pages 3-15
    3. Bryna Rackerby, Sang In Lee, Ian Moppert, Steven C. Ricke, KwangCheol C. Jeong, Si Hong Park
      Pages 17-27
    4. Jennifer L. Endres, Vijaya Kumar Yajjala, Paul D. Fey, Kenneth W. Bayles
      Pages 29-37
    5. Martin Nilsson, Michael Givskov, Tim Tolker-Nielsen
      Pages 39-49
    6. Lorena Ruiz, Douwe van Sinderen
      Pages 51-62
    7. Oindrila Paul, Damayanti Chakravarty, Janet R. Donaldson
      Pages 63-71
    8. Supraja Puttamreddy, F. Chris Minion
      Pages 73-80
    9. Julie L. Stoudenmire, Michael Black, Pat M. Fidopiastis, Eric V. Stabb
      Pages 87-104
    10. Yaligara Veeranagouda, Fasahath Husain, Hannah M. Wexler
      Pages 105-116
    11. John P. Bannantine, Denise K. Zinniel, Raúl G. Barletta
      Pages 117-125
  3. Transposon General Protocols and Applications

    1. Front Matter
      Pages 127-127
    2. Bijay K. Khajanchi, Pravin R. Kaldhone, Steven L. Foley
      Pages 129-139
    3. Yichao Yang, Reena Chandrashekar, Steven C. Ricke, Young Min Kwon
      Pages 141-150
    4. Sarah Delaney, Richard Murphy, Fiona Walsh
      Pages 151-157
    5. Hossam Abdelhamed, Attila Karsi, Mark L. Lawrence
      Pages 159-170
  4. Back Matter
    Pages 181-183

About this book


This book addresses cutting-edge techniques for researching transposon mutagenesis, an approach for identifying individual gene contributions to the phenotypic characteristics of a particular microorganism. The volume begins with methods for specific microorganisms and include protocols for individual microorganisms ranging from pathogens such as Salmonella to Bifidobacterium, a microorganism considered beneficial to humans and animals. The final section addresses more general protocols including plasmid transfer and bioinformatic tools as well as novel applications of transposon methodologies such as transposon-aided capture of antibiotic resistant plasmids. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Microbial Transposon Mutagenesis: Protocols and Applications serves as a valuable reference for scientists seeking to apply transposon mutagenesis to microbial genetic analyses and functionality.


Phenotypic characteristics Transposon mutant libraries Whole genome sequencing Salmonella Microorganism genetics Bifidobacterium Bioinformatic tools

Editors and affiliations

  • Steven C. Ricke
    • 1
  • Si Hong Park
    • 2
  • Morgan L. Davis
    • 3
  1. 1.Department of Food Science, Center for Food SafetyUniversity of ArkansasFayettevilleUSA
  2. 2.Department of Food Science and TechnologyOregon State UniversityCorvallisUSA
  3. 3.Department of Food ScienceUniversity of ArkansasFayettevilleUSA

Bibliographic information

  • Book Title Microbial Transposon Mutagenesis
  • Book Subtitle Protocols and Applications
  • Editors Steven C. Ricke
    Si Hong Park
    Morgan L. Davis
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-9569-1
  • Softcover ISBN 978-1-4939-9572-1
  • eBook ISBN 978-1-4939-9570-7
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XI, 183
  • Number of Illustrations 12 b/w illustrations, 34 illustrations in colour
  • Topics Microbial Genetics and Genomics
  • Buy this book on publisher's site
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