Comparative Embryo Culture

Methods and Protocols

  • Jason R. Herrick

Part of the Methods in Molecular Biology book series (MIMB, volume 2006)

Table of contents

  1. Front Matter
    Pages i-xi
  2. David K. Gardner, Thi T. Truong
    Pages 13-32
  3. Michael T. Kane
    Pages 63-91
  4. Bethany K. Redel, Lee D. Spate, Randall S. Prather
    Pages 93-103
  5. Paula Tríbulo, Rocío Melissa Rivera, Martha Sofia Ortega Obando, Elizabeth Ann Jannaman, Peter J. Hansen
    Pages 115-129
  6. S. Mondal, A. Mor, I. J. Reddy, S. Nandi, P. S. P. Gupta, A. Mishra
    Pages 131-140
  7. Joanna Maria G. Souza-Fabjan, Ribrio Ivan T. P. Batista, Vicente José F. Freitas, Pascal Mermillod
    Pages 141-153
  8. Dolors Izquierdo, María-Gracia Catalá, María-Teresa Paramio
    Pages 155-163
  9. Jennifer P. Barfield
    Pages 165-177
  10. Marcello Rubessa, Lucia Boccia, Serena Di Francesco
    Pages 179-190
  11. Debra K. Berg, Ampika Thongphakdee
    Pages 191-207
  12. Islam M. Saadeldin, Ayman Abdel-Aziz Swelum, Abdullah N. Alowaimer
    Pages 209-218
  13. Elaine M. Carnevale, Lisa J. Maclellan, Jo Anne E. Stokes
    Pages 219-227
  14. Jason R. Herrick
    Pages 229-246
  15. J. B. Nagashima, A. J. Travis, N. Songsasen
    Pages 247-267
  16. Jane C. Fenelon, Bruce D. Murphy
    Pages 269-277
  17. Lynne Selwood
    Pages 279-308
  18. Jenna Kropp Schmidt, Thaddeus G. Golos
    Pages 309-319
  19. Cathy Ramsey, Carol Hanna
    Pages 341-353
  20. Rebecca L. Krisher, Terry Schlenker
    Pages 355-371
  21. Back Matter
    Pages 383-397

About this book


This detailed volume contains embryo culture techniques that should inspire embryologists to consider comparative studies. The species included in this volume represent a broad range of taxa, whose embryos have vastly different culture requirements and developmental characteristics.  Among the species described in this volume, significant differences exist in the rates of cell division, the timing of the maternal to embryonic transition, the relative lipid content of the cytoplasm, the sensitivity of the embryo to specific environmental ions, the preferred nutrients and associated metabolic pathways used by the embryo, the timing and mechanisms of early lineage specification, the presence or absence of embryonic diapause, and the time from fertilization to implantation. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Comparative Embryo Culture: Methods and Protocols serves as an ideal aid for scientists seeking another species whose embryos have evolved a unique mechanism that could provide a valuable, comparative context for experimentation.


Biomedical research Blastocysts Fertility Embryonic development Comparative studies

Editors and affiliations

  • Jason R. Herrick
    • 1
  1. 1.Department of Reproductive SciencesOmaha’s Henry Doorly Zoo and AquariumOmahaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9565-3
  • Online ISBN 978-1-4939-9566-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site