© 2019


Methods and Protocols

  • Johannes Liesche


  • Includes cutting-edge techniques

  • Provides step-by-step detail essential for reproducible results

  • Contains key implementation advice from the experts


Part of the Methods in Molecular Biology book series (MIMB, volume 2014)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Investigating Phloem Anatomy

    1. Front Matter
      Pages 1-1
    2. Pascal Hunziker, Alexander Schulz
      Pages 17-27
    3. Daniel L. Mullendore
      Pages 29-35
    4. Jared J. Stewart, Onno Muller, Christopher M. Cohu, Barbara Demmig-Adams, William W. Adams III
      Pages 55-72
  3. Visualizing Phloem Proteins

    1. Front Matter
      Pages 81-81
    2. Ryan C. Stanfield, Alexander Schulz
      Pages 83-94
    3. Thibaud Cayla, Rozenn Le Hir, Sylvie Dinant
      Pages 95-108
  4. Sampling Phloem Sap

    1. Front Matter
      Pages 123-123
    2. Teemu Paljakka, Anna Lintunen, Yann Salmon, Teemu Hölttä
      Pages 135-142
  5. Measurement of Phloem Transport

    1. Front Matter
      Pages 143-143
    2. Daniel Epron, Masako Dannoura, Caroline Plain
      Pages 145-151
    3. Christopher Vincent, Peter E. H. Minchin, Johannes Liesche
      Pages 153-162
    4. Benjamin A. Babst, Richard Ferrieri, Michael Schueller
      Pages 163-176
    5. Michael R. Thorpe, Peter E. H. Minchin
      Pages 177-194

About this book


This detailed book presents methods that enable the determination of parameters relevant to phloem research in the most efficient ways, putting plant scientists with access to adequate instrumentation in the position to answer any phloem-related question. The collection explores techniques that have been used for decades, such as tracing phloem transport with carbon isotopes, as well as recent developments, such as esculin-based assays of phloem transport and super-resolution microscopy of phloem proteins. As such, the book presents the state-of-the-art in phloem research and, at the same time, a starting point for the development of new methods that will fill the remaining gaps in the phloem researcher’s toolbox in the future. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and comprehensive, Phloem: Methods and Protocols is an ideal guide for all researchers exploring this challenging area of plant biology.


Molecular processes Ecophysiology Plant physiology Theoretical modeling Phloem transport Carbon isotopes Plant development Plant signaling

Editors and affiliations

  • Johannes Liesche
    • 1
  1. 1.College of Life SciencesNorthwest A&F UniversityYanglingChina

Bibliographic information

  • Book Title Phloem
  • Book Subtitle Methods and Protocols
  • Editors Johannes Liesche
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-9561-5
  • Softcover ISBN 978-1-4939-9564-6
  • eBook ISBN 978-1-4939-9562-2
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XIV, 473
  • Number of Illustrations 20 b/w illustrations, 87 illustrations in colour
  • Topics Plant Sciences
  • Buy this book on publisher's site
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