Methods and Protocols

  • Johannes Liesche

Part of the Methods in Molecular Biology book series (MIMB, volume 2014)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Investigating Phloem Anatomy

    1. Front Matter
      Pages 1-1
    2. Pascal Hunziker, Alexander Schulz
      Pages 17-27
    3. Daniel L. Mullendore
      Pages 29-35
    4. Jared J. Stewart, Onno Muller, Christopher M. Cohu, Barbara Demmig-Adams, William W. Adams III
      Pages 55-72
  3. Visualizing Phloem Proteins

    1. Front Matter
      Pages 81-81
    2. Ryan C. Stanfield, Alexander Schulz
      Pages 83-94
    3. Thibaud Cayla, Rozenn Le Hir, Sylvie Dinant
      Pages 95-108
  4. Sampling Phloem Sap

    1. Front Matter
      Pages 123-123
    2. Teemu Paljakka, Anna Lintunen, Yann Salmon, Teemu Hölttä
      Pages 135-142
  5. Measurement of Phloem Transport

    1. Front Matter
      Pages 143-143
    2. Daniel Epron, Masako Dannoura, Caroline Plain
      Pages 145-151
    3. Christopher Vincent, Peter E. H. Minchin, Johannes Liesche
      Pages 153-162
    4. Benjamin A. Babst, Richard Ferrieri, Michael Schueller
      Pages 163-176
    5. Michael R. Thorpe, Peter E. H. Minchin
      Pages 177-194
    6. Jessica A. Savage, Maciej A. Zwieniecki
      Pages 203-211
  6. Investigating Mechanism and Quantities of Phloem Loading and Unloading

    1. Front Matter
      Pages 213-213
    2. Helle Juel Martens, Chen Gao, Johannes Liesche
      Pages 215-221
    3. Umesh P. Yadav, Mearaj A. Shaikh, John Evers, Kamesh C. Regmi, Roberto A. Gaxiola, Brian G. Ayre
      Pages 223-233
  7. Methods to Study Phloem Physiology

    1. Front Matter
      Pages 289-289
    2. Nick Gould, Peter E. H. Minchin, Michael R. Thorpe
      Pages 291-299
  8. Theoretical Models and Microfluidics of Phloem Function

  9. Identifying Phloem Signals

    1. Front Matter
      Pages 409-409
    2. Chao Xia, Yi Zheng, Jing Huang, Zhangjun Fei, Cankui Zhang
      Pages 421-431
    3. Daogang Guan, Yiji Xia, Shoudong Zhang
      Pages 433-438
    4. Matthias R. Zimmermann, Jens B. Hafke, Maria K. Paulmann, Alexandra C. U. Furch
      Pages 439-447
    5. Alexandra C. U. Furch, Matthias R. Zimmermann, Torsten Will
      Pages 449-457
  10. Back Matter
    Pages 467-473

About this book


This detailed book presents methods that enable the determination of parameters relevant to phloem research in the most efficient ways, putting plant scientists with access to adequate instrumentation in the position to answer any phloem-related question. The collection explores techniques that have been used for decades, such as tracing phloem transport with carbon isotopes, as well as recent developments, such as esculin-based assays of phloem transport and super-resolution microscopy of phloem proteins. As such, the book presents the state-of-the-art in phloem research and, at the same time, a starting point for the development of new methods that will fill the remaining gaps in the phloem researcher’s toolbox in the future. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and comprehensive, Phloem: Methods and Protocols is an ideal guide for all researchers exploring this challenging area of plant biology.


Molecular processes Ecophysiology Plant physiology Theoretical modeling Phloem transport Carbon isotopes Plant development Plant signaling

Editors and affiliations

  • Johannes Liesche
    • 1
  1. 1.College of Life SciencesNorthwest A&F UniversityYanglingChina

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9561-5
  • Online ISBN 978-1-4939-9562-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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