© 2019

Enzyme-Mediated Ligation Methods

  • Timo Nuijens
  • Marcel Schmidt


  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts


Part of the Methods in Molecular Biology book series (MIMB, volume 2012)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Rémy Gébleux, Manfred Briendl, Ulf Grawunder, Roger R. Beerli
    Pages 1-13
  3. Akello J. Agwa, David J. Craik, Christina I. Schroeder
    Pages 29-41
  4. Xinya Hemu, Xiaohong Zhang, Xiaobao Bi, Chuan-Fa Liu, James P. Tam
    Pages 83-109
  5. Sandra Liebscher, Frank Bordusa
    Pages 111-133
  6. Stephan Dickgiesser, Lukas Deweid, Roland Kellner, Harald Kolmar, Nicolas Rasche
    Pages 135-149
  7. Lukas Deweid, Olga Avrutina, Harald Kolmar
    Pages 151-169
  8. Vanessa Siegmund, Birgit Piater, Frank Fischer, Harald Kolmar
    Pages 171-192
  9. Karen S. Harris, Simon Poon, Pedro Quimbar, Marilyn A. Anderson
    Pages 211-235
  10. Jan Grünewald, Ansgar Brock, Bernhard H. Geierstanger
    Pages 237-278
  11. Rhiannon M. Sears, Danielle G. May, Kyle J. Roux
    Pages 299-313
  12. Marcus Gerlach, Tina Stoschek, Heinrich Leonhardt, Christian P. R. Hackenberger, Dominik Schumacher, Jonas Helma
    Pages 327-355
  13. Jorick J. Bruins, Criss van de Wouw, Jordi F. Keijzer, Bauke Albada, Floris L. van Delft
    Pages 357-368
  14. Back Matter
    Pages 369-371

About this book


This volume discusses different enzyme-catalyzed ligation methodologies for a variety of different chemical transformations. This book wants readers to view enzymes as a powerful tool in both academic and industrial research. Chapters in this book cover topics such as sortase A-mediated generation of site-specifically conjugated antibody-drug conjugates; omniligase-catalyzed inter- and intramolecular ligation; ligation catalyzed by microbial transglutaminase; peptide cyclization mediated by cyanobactin macrocyclases, butelase 1 and sortase A; using BioID as a tool for protein proximity labeling in living cells; and inducible, selective labeling of proteins via enzymatic oxidation of tyrosine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Enzyme-Mediated Ligation Methods is a valuable resource for students and scientists from different disciplines who are interested in using enzymatic strategies to answer their research questions. 


biotherapeutics phosphopantheinyl transferase Glutathione-S-Transferase Peptide Coupling cyclization

Editors and affiliations

  • Timo Nuijens
    • 1
  • Marcel Schmidt
    • 2
  1. 1.EnzyPep B.V.GeleenThe Netherlands
  2. 2.EnzyPep B.V.GeleenThe Netherlands

Bibliographic information

  • Book Title Enzyme-Mediated Ligation Methods
  • Editors Timo Nuijens
    Marcel Schmidt
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-9545-5
  • Softcover ISBN 978-1-4939-9548-6
  • eBook ISBN 978-1-4939-9546-2
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XI, 371
  • Number of Illustrations 35 b/w illustrations, 59 illustrations in colour
  • Topics Biotechnology
    Protein-Ligand Interactions
  • Buy this book on publisher's site
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