Pathogenic Yersinia

1. Yersinia Interaction with Immune Cells and Immune Signaling

   1. Analysis of Inflammasome Activation in Response to Yersinia Infection by Fluorescence Microscopy Detection of Active Caspase-1 Puncta

      • Erin E. Zwack, Igor E. Brodsky

      Pages 231-240

   2. Methods for Detection of Pyrin Inflammasome Assembly in Macrophages Infected with Yersinia spp.

      • Natasha P. Medici, James B. Bliska

      Pages 241-255

2. Back Matter

   Pages 257-259

   PDF

This volume delivers a compendium of detailed protocols to the research community in order to aid in the investigation and discovery of Yersinia virulence mechanisms using important in vivo and in vitro infection models, which have led to major advances in the field and in our understanding of Yersinia pathogen-host interactions. Beginning with a section on mouse models, the book continues with chapters covering the monitoring of bacteria during infection, invertebrate models, as well as Yersinia interaction with immune cells and immune signaling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 

Comprehensive and authoritative, Pathogenic Yersinia: Methods and Protocols providesa single source for researchers seeking to better understand these pathogens and the diseases they produce in humans.

• Virulence mechanisms
• Infection models
• Host-pathogen interactions
• Mouse models
• Bacterial proliferation
• Immune response
• infectious diseases

“The book is written for the research community, including novices. Clinicians and parasitologists with an interest in investigation and discovery of Yersinia virulence mechanisms may also find this book helpful and interesting. … This is a wonderful resource for understanding the investigation and discovery of the virulence mechanisms of Yersinia. … This book is quite unique in covering the methods and protocols related to Yersinia pathogen‐host interactions.” (Omer Iqbal, Doody's Book Reviews, November 01, 2019)

• Paul G Allen School for Global Animal Health, Washington State University, Pullman, USA

  Viveka Vadyvaloo

• Department of Microbiolgy and Immunology and Center for Predictive Medicine in Biodefense and Emerging Infectious Diseases, University of Louisville School of Medicine, Louisville, USA

  Matthew B. Lawrenz

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