© 2019

Pathogenic Yersinia

Methods and Protocols

  • Viveka Vadyvaloo
  • Matthew B. Lawrenz
  • Includes cutting-edge techniques

  • Provides step-by-step detail essential for reproducible results

  • Contains key implementation advice from the experts


Part of the Methods in Molecular Biology book series (MIMB, volume 2010)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Mouse Models for Yersinia Infection

    1. Front Matter
      Pages 1-1
    2. Paul E. Anderson, Rachel M. Olson, Joshua L. Willix, Deborah M. Anderson
      Pages 29-39
    3. Diana Hooker-Romero, Leah Schwiesow, Yahan Wei, Victoria Auerbuch
      Pages 41-53
  3. Monitoring Bacteria During Infection

    1. Front Matter
      Pages 55-55
    2. Anne Derbise, Olivier Dussurget, Elisabeth Carniel, Javier Pizarro-Cerdá
      Pages 85-97
    3. Maria Kusmierek, Ann Kathrin Heroven, Michael Beckstette, Aaron M. Nuss, Petra Dersch
      Pages 99-116
    4. Giang T. Nguyen, Anne L. McCabe, Alyssa C. Fasciano, Joan Mecsas
      Pages 117-139
    5. Yaron Vagima, Yinon Levy, Emanuelle Mamroud
      Pages 141-150
  4. Invertebrate Models of Infection (In Vivo and In Vitro)

    1. Front Matter
      Pages 151-151
    2. Athena Lemon, Amelia Silva-Rohwer, Janelle Sagawa, Viveka Vadyvaloo
      Pages 153-166
    3. Anna B. Ludlow, Cassandra D. Pauling, Melanie M. Marketon, Deborah M. Anderson
      Pages 167-178
  5. Yersinia Interaction with Immune Cells and Immune Signaling

    1. Front Matter
      Pages 179-179
    2. Amanda R. Pulsifer, Tiva T. VanCleave, Matthew B. Lawrenz
      Pages 181-196
    3. Kara R. Eichelberger, William E. Goldman
      Pages 197-209
    4. Tiago R. D. Costa, Monika K. Francis, Salah I. Farag, Tomas Edgren, Matthew S. Francis
      Pages 211-229

About this book


This volume delivers a compendium of detailed protocols to the research community in order to aid in the investigation and discovery of Yersinia virulence mechanisms using important in vivo and in vitro infection models, which have led to major advances in the field and in our understanding of Yersinia pathogen-host interactions. Beginning with a section on mouse models, the book continues with chapters covering the monitoring of bacteria during infection, invertebrate models, as well as Yersinia interaction with immune cells and immune signaling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 

Comprehensive and authoritative, Pathogenic Yersinia: Methods and Protocols provides a single source for researchers seeking to better understand these pathogens and the diseases they produce in humans.


Virulence mechanisms Infection models Host-pathogen interactions Mouse models Bacterial proliferation Immune response

Editors and affiliations

  • Viveka Vadyvaloo
    • 1
  • Matthew B. Lawrenz
    • 2
  1. 1.Paul G Allen School for Global Animal HealthWashington State UniversityPullmanUSA
  2. 2.Department of Microbiolgy and Immunology and Center for Predictive Medicine in Biodefense and Emerging Infectious DiseasesUniversity of Louisville School of MedicineLouisvilleUSA

Bibliographic information

  • Book Title Pathogenic Yersinia
  • Book Subtitle Methods and Protocols
  • Editors Viveka Vadyvaloo
    Matthew B. Lawrenz
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-9540-0
  • Softcover ISBN 978-1-4939-9543-1
  • eBook ISBN 978-1-4939-9541-7
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XI, 259
  • Number of Illustrations 6 b/w illustrations, 40 illustrations in colour
  • Topics Bacteriology
    Infectious Diseases
  • Buy this book on publisher's site
Industry Sectors


“The book is written for the research community, including novices. Clinicians and parasitologists with an interest in investigation and discovery of Yersinia virulence mechanisms may also find this book helpful and interesting. … This is a wonderful resource for understanding the investigation and discovery of the virulence mechanisms of Yersinia. … This book is quite unique in covering the methods and protocols related to Yersinia pathogen‐host interactions.” (Omer Iqbal, Doody's Book Reviews, November 01, 2019)