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DNA Repair

Methods and Protocols

  • Lata Balakrishnan
  • Jason A. Stewart
Book

Part of the Methods in Molecular Biology book series (MIMB, volume 1999)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Reviews

    1. Front Matter
      Pages 1-1
    2. Emily Mason-Osann, Himabindu Gali, Rachel Litman Flynn
      Pages 31-57
  3. Cellular Assays to Detect and Measure DNA Damage and Damage Response

    1. Front Matter
      Pages 59-59
    2. Jae Jin Kim, Ramhari Kumbhar, Fade Gong, Kyle M. Miller
      Pages 61-74
    3. Yanhao Lai, Ruipeng Lei, Yaou Ren, Yuan Liu
      Pages 87-101
    4. Radha Subramaniam, Natalie A. Lamb, Yoonchan Hwang, Lauren Johengen, Jennifer A. Surtees
      Pages 103-127
  4. Cellular and Cell Extract-Based Assays to Measure DNA Repair

    1. Front Matter
      Pages 129-129
    2. Dorota Piekna-Przybylska
      Pages 145-160
    3. Sanket Awate, Srijita Dhar, Joshua A. Sommers, Robert M. Brosh Jr.
      Pages 185-207
    4. John J. Turchi, Pamela S. VanderVere-Carozza
      Pages 217-221
  5. In Vitro Biochemical and Biophysical Methods to Study DNA Repair

    1. Front Matter
      Pages 223-223
    2. Michael M. Soniat, Logan R. Myler, Ilya J. Finkelstein
      Pages 225-244
    3. Christopher W. Sausen, Cody M. Rogers, Matthew L. Bochman
      Pages 245-253
    4. Cody M. Rogers, Christopher W. Sausen, Matthew L. Bochman
      Pages 255-264
    5. Steven D. Goodson, Russell B. Hawes, Sarah M. Waldvogel, Michael G. Sehorn
      Pages 265-270
    6. Andrew A. Kelso, Steven D. Goodson, Michael G. Sehorn
      Pages 271-284
    7. Sarah M. Waldvogel, Steven D. Goodson, Michael G. Sehorn
      Pages 285-291
  6. Methods to Assess Telomeric and Replication-Induced DNA Damage and Repair

    1. Front Matter
      Pages 293-293
    2. Elise Fouquerel, Ryan P. Barnes, Hong Wang, Patricia L. Opresko
      Pages 295-306
    3. Srijita Dhar, Arindam Datta, Taraswi Banerjee, Robert M. Brosh Jr.
      Pages 307-318
    4. Himabindu Gali, Emily Mason-Osann, Rachel Litman Flynn
      Pages 319-325
    5. P. Logan Schuck, Jason A. Stewart
      Pages 335-347
  7. Back Matter
    Pages 349-352

About this book

Introduction

The volume outlines techniques used to study the primary mechanisms of important DNA repair pathways. The chapters in this book are organized into five sections, each discussing a specific aspect of repair biology. Part I reviews the role of post-translational modifications of HDR, and DNA damage caused by defective telomere replication. Part II describes methods to detect and measure DNA breaks and proteins involved in DNA damage response. Part III talks about methods designed to measure DNA repair efficiency and characterize factors involved in the repair process. Part IV discusses various in vitro assays used to purify recombinant proteins that are used to study DNA repair enzyme mechanisms. Lastly, Part V focuses on methods to study the damage arising during DNA replication. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, DNA Repair: Methods and Protocols is an essential resource for novice and experienced researchers, and provides easily adaptable techniques to help them advance in their specific protein or repair pathway of interest.

Keywords

CRISPR Laser microradiation Xenopus nucleosomal templates ATPase assays

Editors and affiliations

  • Lata Balakrishnan
    • 1
  • Jason A. Stewart
    • 2
  1. 1.Indiana University – Purdue UniversityIndianapolisUSA
  2. 2.Department of Biological SciencesUniversity of South CarolinaColumbiaUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-9500-4
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9499-1
  • Online ISBN 978-1-4939-9500-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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