Neisseria gonorrhoeae

Methods and Protocols

  • Myron Christodoulides

Part of the Methods in Molecular Biology book series (MIMB, volume 1997)

Table of contents

  1. Front Matter
    Pages i-xvii
  2. Jutamas Shaughnessy, Sanjay Ram, Peter A. Rice
    Pages 1-27
  3. Stephanie E. McLaughlin, J. McLeod Griffiss
    Pages 29-36
  4. Magnus Unemo, Daniel Golparian, David W. Eyre
    Pages 37-58
  5. John E. Heckels
    Pages 77-85
  6. Lisa Craig, Tuba Altindal
    Pages 97-110
  7. Ryan E. Schaub, Joseph P. Dillard
    Pages 111-120
  8. Ian P. Francis, Xiuping Lui, Lee M. Wetzler
    Pages 121-141
  9. Melanie M. Callaghan, Joseph P. Dillard
    Pages 143-162
  10. Fadi E. El-Rami, Aleksandra E. Sikora
    Pages 185-205
  11. Sunita Gulati, Peter A. Rice, Sanjay Ram
    Pages 267-280
  12. Hannia Liliana Almonacid-Mendoza, Myron Christodoulides
    Pages 281-299
  13. Jimena Gatica, Paula I. Rodas, Alejandro Escobar
    Pages 301-318
  14. Colin P. Churchward, Lori A. S. Snyder
    Pages 363-376
  15. A. Said Álamos-Musre, Alejandro Escobar, Cecilia V. Tapia, Myron Christodoulides, Paula I. Rodas
    Pages 377-402
  16. Katherine Rhodes, Mancheong Ma, Magdalene So
    Pages 403-412
  17. Erica L. Raterman, Ann E. Jerse
    Pages 413-429
  18. Marcia M. Hobbs, Joseph A. Duncan
    Pages 431-452
  19. Back Matter
    Pages 453-461

About this book


This detailed book addresses the continual development and application of new methods and protocols to understand the biology of the pathogen Neisseria gonorrhoeae. Beginning with gonococcus biology and antimicrobial resistance, the volume continues with areas of study such as producing major gonococcal antigens to high purity, genetic transformation of gonococci and strategies for global RNA sequencing, evaluation of potential vaccine/drug targets and the assessment of hypothetical protein function, as well as a number of methods and protocols for studying the biological interactions of the gonococcus and the host. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Comprehensive and cutting-edge, Neisseria gonorrhoeae: Methods and Protocols provides researchers with a foundation for progress toward the eventual control of this disease and a significant reduction in global case numbers of gonorrhea.


Host-pathogen interactions Antimicrobial resistance Gonococci Drug targets Cell culture models Vaccine development

Editors and affiliations

  • Myron Christodoulides
    • 1
  1. 1.Molecular Microbiology Group, Academic Unit of Clinical and Experimental Sciences, Faculty of MedicineUniversity of SouthamptonSouthamptonUK

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9495-3
  • Online ISBN 978-1-4939-9496-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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