© 2019

The ESCRT Complexes

Methods and Protocols

  • Emmanuel Culetto
  • Renaud Legouis
  • Includes cutting-edge techniques

  • Provides step-by-step detail essential for reproducible results

  • Contains key implementation advice from the experts


Part of the Methods in Molecular Biology book series (MIMB, volume 1998)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Rachel Y. Samson, Iain G. Duggin, Stephen D. Bell
    Pages 1-11
  3. Tamás Matusek, Pascal Thérond, Maximilian Fürthauer
    Pages 31-47
  4. Binod Kumar, Mohanan Valiya Veettil, Arunava Roy, Bala Chandran
    Pages 63-72
  5. Keisuke Tabata, Atsuki Nara, Hiroko Omori, Eiji Morita
    Pages 73-92
  6. Jalal M. Kazan, Gergely L. Lukacs, Pirjo M. Apaja, Arnim Pause
    Pages 93-103
  7. Sudeep Banjade, Shaogeng Tang, Scott D. Emr
    Pages 105-116
  8. Veronica Birdsall, Jose C. Martinez, Lisa Randolph, Ulrich Hengst, Clarissa L. Waites
    Pages 117-128
  9. Romain Le Bars, Michele W. Bianchi, Christophe Lefebvre
    Pages 149-161
  10. Samuel Block, Amber L. Schuh, Anjon Audhya
    Pages 189-202
  11. Luisa Capalbo, Ioanna Mela, Maria Alba Abad, A. Arockia Jeyaprakash, J. Michael Edwardson, Pier Paolo D’Avino
    Pages 203-217
  12. Francheska Son, Katharine Umphred-Wilson, Jae-Hyuck Shim, Stanley Adoro
    Pages 219-226
  13. Julio Paez-Valencia, Marisa S. Otegui
    Pages 227-238
  14. Imane M. Rezig, Shaun K. Bremner, Musab S. Bhutta, Ian P. Salt, Gwyn W. Gould, Christopher J. McInerny
    Pages 239-250
  15. Carl Haag, Thomas Klein, Michael Feldbrügge
    Pages 251-271

About this book


This detailed collection gathers both established and recent technical procedures to study the Endosomal Sorting Complex Required for Transport (ESCRT) complexes in a wide range of biological systems: Archaea, A. thaliana, U. maydis, S. cerevisiae, S. pombe, C. elegans, D. melanogaster, and mammalian cells. Opening with a section on imaging techniques, the book continues with chapters covering biochemical approaches presenting strategies for production and characterization of recombinant ESCRT proteins, or of specific ESCRT protein domains, as well as genetic and proteomic experimental approaches. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, The ESCRT Complexes: Methods and Protocols serves as a compact guide for researchers interested in establishing an integrated approach to investigate the ESCRT machinery functions in cell biology.


Endosomal Sorting Complexes Required for Transport ESCRT proteins Imaging techniques Membrane structures Genetic approaches

Editors and affiliations

  • Emmanuel Culetto
    • 1
  • Renaud Legouis
    • 2
  1. 1.Institute for Integrative Biology of the Cell (I2BC)CEA, CNRS, Univ.Paris-Sud, Université Paris-SaclayGif-sur-YvetteFrance
  2. 2.Institute for Integrative Biology of the Cell (I2BC)CEA, CNRS, Univ.Paris-Sud, Université Paris-SaclayGif-sur-YvetteFrance

Bibliographic information

  • Book Title The ESCRT Complexes
  • Book Subtitle Methods and Protocols
  • Editors Emmanuel Culetto
    Renaud Legouis
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-9491-5
  • Softcover ISBN 978-1-4939-9494-6
  • eBook ISBN 978-1-4939-9492-2
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XIV, 322
  • Number of Illustrations 29 b/w illustrations, 43 illustrations in colour
  • Topics Membrane Biology
    Protein Science
    Cell Physiology
  • Buy this book on publisher's site