Skin Tissue Engineering

Methods and Protocols

  • Sophie Böttcher-Haberzeth
  • Thomas Biedermann

Part of the Methods in Molecular Biology book series (MIMB, volume 1993)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Isolation and Culture of Skin Cells

    1. Front Matter
      Pages 1-1
    2. Sergio Cortez Ghio, Gaëtan Le-Bel, Amélie Lavoie, Danielle Larouche, Lucie Germain
      Pages 3-13
    3. Daisuke Suzuki, Filipa Pinto, Makoto Senoo
      Pages 15-31
    4. Muriel Cario, Alain Taieb
      Pages 33-46
    5. Dongrui Ma, Seng-Teik Lee, Alvin Wen Choong Chua
      Pages 61-70
    6. Marta A. Kisiel, Agnes S. Klar
      Pages 71-78
    7. Jennifer Bourland, Dominique Mayrand, Nathalie Tremblay, Véronique J. Moulin, Julie Fradette, François A. Auger
      Pages 79-90
    8. Joris A. van Dongen, Martin C. Harmsen, Hieronymus P. Stevens
      Pages 91-103
  3. Tissue Engineering Skin and Engineered Skin Models

    1. Front Matter
      Pages 105-105
    2. Lydia Costello, Nicola Fullard, Mathilde Roger, Steven Bradbury, Teresa Dicolandrea, Robert Isfort et al.
      Pages 107-122
    3. Takamitsu Matsuzawa, Michiyo Nakano, Ayako Oikawa, Yuumi Nakamura, Hiroyuki Matsue
      Pages 123-137
    4. Andrea L. Lalley, Steven T. Boyce
      Pages 159-179
  4. In Vitro and Vivo Models Testing Skin, Skin Cells, and Tissue Engineered Skin

    1. Front Matter
      Pages 191-191
    2. Jiang-Hui Wang, Jinying Chen, Shyh-Ming Kuo, Geraldine M. Mitchell, Shiang Y. Lim, Guei-Sheung Liu
      Pages 217-226
    3. Jun Mi, Shuai Chen, Lin Xu, Jie Wen, Xin Xu, Xunwei Wu
      Pages 227-237
    4. Christian Tapking, Daniel Popp, Ludwik K. Branski
      Pages 239-249
    5. Thea Fleischmann, Flora Nicholls, Miriam Lipiski, Margarete Arras, Nikola Cesarovic
      Pages 251-259
  5. Back Matter
    Pages 261-263

About this book


This volume provides protocols describing the isolation and culture of diverse cell types stemming from the skin and the use of these cells and cell constructs for wound healing, bioengineering applications, and translational medicine purposes. The book is divided into three sections describing the isolation and culture of diverse skin cells, managing these cells within co-culture systems and skin models, as well as using these skin models in a test setting. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Skin Tissue Engineering: Methods and Protocols serves as a vital aid to basic and clinical researchers such as biologists, physicians, and biomedical engineers working with and being interested in basic science, and clinically and laboratory-applicable translational regenerative medicine.


Skin models Wound repair Biomedical engineering Translational medicine Cell isolation

Editors and affiliations

  • Sophie Böttcher-Haberzeth
    • 1
  • Thomas Biedermann
    • 2
  1. 1.University Children’s Hospital ZurichZurichSwitzerland
  2. 2.University Children’s Hospital ZurichZurichSwitzerland

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9472-4
  • Online ISBN 978-1-4939-9473-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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