Mass Cytometry

Methods and Protocols

  • Helen M. McGuire
  • Thomas M. Ashhurst

Part of the Methods in Molecular Biology book series (MIMB, volume 1989)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Setting Up a Facility

    1. Front Matter
      Pages 1-1
    2. Sarah Warth, Désirée Kunkel
      Pages 3-11
  3. Panel Design and Reagent Preparation

    1. Front Matter
      Pages 33-33
    2. Amelia Au-Yeung, Chikara Takahashi, W. Rodney Mathews, William E. O’Gorman
      Pages 35-45
    3. Stephen Gregory Chang, Cynthia J. Guidos
      Pages 47-54
    4. Felix J. Hartmann, Erin F. Simonds, Nora Vivanco, Trevor Bruce, Luciene Borges, Garry P. Nolan et al.
      Pages 55-81
    5. Caryn van Vreden, Paula Niewold, Helen M. McGuire, Barbara Fazekas de St. Groth
      Pages 83-92
    6. Axel Ronald Schulz, Henrik E. Mei
      Pages 93-108
  4. Sample Preparation

    1. Front Matter
      Pages 109-109
    2. Jaromir Mikes, Axel Olin, Tadepally Lakshmikanth, Yang Chen, Petter Brodin
      Pages 111-123
  5. Specific Applications

    1. Front Matter
      Pages 137-137
    2. Diana Shinko, Thomas M. Ashhurst, Helen M. McGuire, Kellie A. Charles
      Pages 139-146
    3. Yannick Simoni, Michael Fehlings, Evan W. Newell
      Pages 147-158
    4. Thomas M. Ashhurst, Darren A. Cox, Adrian L. Smith, Nicholas J. C. King
      Pages 159-192
    5. Gregory K. Behbehani
      Pages 193-215
    6. Mikael Roussel, Todd Bartkowiak, Jonathan M. Irish
      Pages 217-226
    7. Andrew J. Mitchell, Angela Ivask, Yi Ju
      Pages 227-241
  6. Data Analysis

    1. Front Matter
      Pages 243-243
    2. Sherrie Wang, Ryan R. Brinkman
      Pages 245-265
    3. Christina B. Pedersen, Lars R. Olsen
      Pages 267-279
    4. Hannah G. Polikowsky, Katherine A. Drake
      Pages 309-332
  7. Back Matter
    Pages 333-338

About this book


This book details a compilation of up-to-date and cutting-edge protocols in mass cytometry.. Chapters guide readers through setting up a facility, panel design and reagent preparation, sample preparation, specific applications, and data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Mass Cytometry: Methods and Protocols aims to ensure successful results in the further study of this vital field.


multiparametric data panel designs phenotyping mouse samples Nanoparticles

Editors and affiliations

  • Helen M. McGuire
    • 1
  • Thomas M. Ashhurst
    • 2
  1. 1.Discipline of PathologyThe University of SydneyCamperdownAustralia
  2. 2.Sydney Cytometry FacilityThe University of Sydney and Centenary InstituteCamperdownAustralia

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9453-3
  • Online ISBN 978-1-4939-9454-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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