Chiral Separations

1. Enantioseparation by Capillary Electrophoresis Using Cyclodextrins in an Amino Acid-Based Ionic Liquid Running Buffer

   • Joachim Wahl, Ulrike Holzgrabe

   Pages 365-371

2. Enantioseparations in Nonaqueous Capillary Electrophoresis Using Charged Cyclodextrins

   • Anne-Catherine Servais, Marianne Fillet

   Pages 373-381

3. Chiral Separation by NACE Using Polyol Derivative–Boric Acid Complexes

   • Lijuan Wang, Xu Hou, Fan Zhang, Ying Liu, Yimeng Ren, Hongyuan Yan

   Pages 383-389

4. Chiral Capillary Electrophoresis-Mass Spectrometry

   • María Castro-Puyana, María Luisa Marina

   Pages 391-405

5. Enantioseparation of Selected Imidazole Drugs Using Dual Cyclodextrin-Modified Micellar Electrokinetic Chromatography

   • Wan Aini Wan Ibrahim, Siti Munirah Abd Wahib, Dadan Hermawan, Mohd Marsin Sanagi

   Pages 407-416

6. Carbohydrate-Based Polymeric Surfactants for Chiral Micellar Electrokinetic Chromatography (CMEKC) Coupled to Mass Spectrometry

   • Vijay Patel, Shahab A. Shamsi

   Pages 417-444

7. Application of an (18-Crown-6)-2,3,11,12-Tetracarboxylic Acid-Based Chiral Stationary Phase in Capillary Electrochromatography

   • Wonjae Lee, Kyung Tae Kim, Jong Seong Kang

   Pages 445-452

8. Experimental Design Methodologies for the Optimization of Chiral Separations: An Overview

   • Luiz Carlos Klein-Júnior, Debby Mangelings, Yvan Vander Heyden

   Pages 453-478

9. Back Matter

   Pages 479-487

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This detailed book focuses on analytical separations by chromatographic and electrophoretic techniques, providing some overview along with numerous practically-oriented applications of the most important analytical techniques in chiral separation sciences. While some compounds may only be enantioseparated with one technique based on the physico-chemical properties, often the analyst can choose between two or more analytical techniques for a given analyte, which requires knowledge of the strengths and weaknesses of each technique in order to select the most appropriate method for the given problem. This collection binds that knowledge in one volume. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Chiral Separations: Methods and Protocols, Third Edition serves as a helpful guide for analytical chemists working on stereochemical problems in the fields of pharmacy, chemistry, biochemistry, food chemistry, molecular biology, forensics, environmental sciences, or cosmetics in academia, government, or industry.

• Liquid Chromatography
• Electrophoretic techniques
• Enantioseparations
• Chiral compounds
• Enantiomers
• Analytical separations

“The book is aimed at investigators in any discipline working in the field of chirality. It will be of special interest to anyone who has a need to separate chiral molecules. … Detailed protocols are provided for the systems covered in the book that will make it easier for investigators wishing to replicate one of these procedures.” (Thomas J. Wenzel, Analytical and Bioanalytical Chemistry, Vol. 411, 2019)

• Department of Pharmaceutical Chemistry, University of Jena, Jena, Germany

  Gerhard K. E. Scriba

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