© 2019

NADPH Oxidases

Methods and Protocols

  • Ulla G. Knaus
  • Thomas L. Leto


  • Includes cutting-edge techniques

  • Provides step-by-step detail essential for reproducible results

  • Contains key implementation advice from the experts


Part of the Methods in Molecular Biology book series (MIMB, volume 1982)

Table of contents

  1. Front Matter
    Pages i-xv
  2. The NADPH Oxidase Family: Overviews

    1. Front Matter
      Pages 1-1
    2. Hélène Buvelot, Vincent Jaquet, Karl-Heinz Krause
      Pages 17-36
  3. Biochemistry and Model Systems

    1. Front Matter
      Pages 37-37
    2. Algirdas J. Jesaitis, Marcia Riesselman, Ross M. Taylor, Susan Brumfield
      Pages 39-59
    3. Tania Bizouarn, Hager Souabni, Xavier Serfaty, Aicha Bouraoui, Rawand Masoud, Gilda Karimi et al.
      Pages 75-101
    4. Fiona Augsburger, Delphine Rasti, Yves Cambet, Vincent Jaquet
      Pages 103-111
    5. Yoko Nakano, William M. Nauseef
      Pages 113-120
    6. Hideki Sumimoto, Reiko Minakami, Kei Miyano
      Pages 121-137
    7. Sylvain Beaumel, Marie José Stasia
      Pages 153-171
    8. Agnieszka Korzeniowska, Ágnes P. Donkó, Stanislas Morand, Thomas L. Leto
      Pages 173-190
    9. Becky A. Diebold, S. Garrett Wilder, Xavier De Deken, Jennifer L. Meitzler, James H. Doroshow, James W. McCoy et al.
      Pages 191-229
  4. Detection of ROS

    1. Front Matter
      Pages 231-231
    2. Fiona Augsburger, Aleksandra Filippova, Vincent Jaquet
      Pages 233-241
    3. Yulia G. Ermakova, Nataliya M. Mishina, Carsten Schultz, Vsevolod V. Belousov
      Pages 259-274

About this book


This detailed volume explores the NADPH oxidase family of enzymes in human physiology and genetic disease, in which early discoveries represent prime examples of the finest translational “from bed to bench and back” studies. Methods are included for testing assembly and function of multicomponent oxidase complexes and for analyzing reactive oxygen species (ROS) generation in different systems by various means, while addressing pitfalls of ROS probes currently being used, as well as protocols on NADPH oxidase regulation and their function in cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, NADPH Oxidases: Methods and Protocols will aid researchers working with the NOX/DUOX family in continuing and expanding upon their vital research.


NOX/DUOX family Multicomponent oxidase complexes Genetic diseases Reactive oxygen species Oxidase regulation Disease modeling

Editors and affiliations

  • Ulla G. Knaus
    • 1
  • Thomas L. Leto
    • 2
  1. 1.Conway Institute, School of MedicineUniversity College DublinDublinIreland
  2. 2.Laboratory of Clinical Immunology and MicrobiologyNational Institute of Allergy and Infectious Diseases, National Institutes of HealthBethesdaUSA

Bibliographic information

  • Book Title NADPH Oxidases
  • Book Subtitle Methods and Protocols
  • Editors Ulla G. Knaus
    Thomas L. Leto
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-9423-6
  • Softcover ISBN 978-1-4939-9426-7
  • eBook ISBN 978-1-4939-9424-3
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XV, 718
  • Number of Illustrations 44 b/w illustrations, 111 illustrations in colour
  • Topics Enzymology
    Human Physiology
    Human Genetics
  • Buy this book on publisher's site
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