High-Throughput Metabolomics

Methods and Protocols

  • Angelo D'Alessandro

Part of the Methods in Molecular Biology book series (MIMB, volume 1978)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Methods

    1. Front Matter
      Pages 1-1
    2. Sarah Hayton, Robert D. Trengove, Garth L. Maker
      Pages 3-12
    3. Travis Nemkov, Julie A. Reisz, Sarah Gehrke, Kirk C. Hansen, Angelo D’Alessandro
      Pages 13-26
    4. Raúl González-Domínguez, Álvaro González-Domínguez, Carmen Segundo, Mónica Schwarz, Ana Sayago, Rosa María Mateos et al.
      Pages 27-38
    5. Giuseppe Paglia, Giuseppe Astarita
      Pages 39-53
    6. Ángeles López-Gonzálvez, Joanna Godzien, Antonia García, Coral Barbas
      Pages 55-77
  3. Lipidomics

    1. Front Matter
      Pages 79-79
    2. Simona Zarini, Robert M. Barkley, Miguel A. Gijón, Robert C. Murphy
      Pages 81-105
    3. Julie A. Reisz, Connie Zheng, Angelo D’Alessandro, Travis Nemkov
      Pages 121-135
  4. Metabolomics of Animal and Plant Models

    1. Front Matter
      Pages 153-153
    2. Federica Gevi, Giuseppina Fanelli, Lello Zolla, Sara Rinalducci
      Pages 187-195
  5. Tracing Experiments and Metabolic Flux Analysis

    1. Front Matter
      Pages 197-197
    2. Gretchen L. Seim, Emily C. Britt, Jing Fan
      Pages 199-217
    3. Reihaneh Mostolizadeh, Andreas Dräger, Neema Jamshidi
      Pages 243-258
    4. Joanna Kalucka, Bart Ghesquière, Sarah-Maria Fendt, Peter Carmeliet
      Pages 259-268
    5. Sara Violante, Mirela Berisa, Tiffany H. Thomas, Justin R. Cross
      Pages 269-283
  6. Data Processing in Metabolomics

    1. Front Matter
      Pages 285-285
    2. Linxing Yao, Amy M. Sheflin, Corey D. Broeckling, Jessica E. Prenni
      Pages 287-299
    3. Shubhra Agrawal, Sahil Kumar, Raghav Sehgal, Sabu George, Rishabh Gupta, Surbhi Poddar et al.
      Pages 301-321
    4. Dominik Reinhold, Harrison Pielke-Lombardo, Sean Jacobson, Debashis Ghosh, Katerina Kechris
      Pages 323-340
  7. Metabolic Measurements with Techniques Orthogonal to Mass Spectrometry

    1. Front Matter
      Pages 341-341
    2. Natalie J. Serkova, Denise M. Davis, Jenna Steiner, Rajesh Agarwal
      Pages 369-387
  8. Towards Personalized Metabolomics

    1. Front Matter
      Pages 389-389
    2. Evagelia C. Laiakis
      Pages 391-402
    3. Pol Herrero, Miguel Ángel Rodríguez, Maria Rosa Ras, Antoni del Pino, Lluís Arola, Núria Canela
      Pages 403-430
    4. David P. Marciano, Michael P. Snyder
      Pages 447-456
  9. Back Matter
    Pages 457-459

About this book


This detailed volume focuses on recent technological, computational, and biostatistical advances in the field of high-throughput metabolomics. Chapters encompass methods, platforms, and analytical strategies for steady state measurements and metabolic flux analysis with stable isotope-labeled tracers, in biological matrices of clinical relevance and model organisms. Mass spectrometry-based or orthogonal methods are discussed, along with computational and statistical methods to address data sparsity in high-throughput metabolomics approaches. As a part of the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, High-Throughput Metabolomics: Methods and Protocols provides tools that can bring about the next generation of clinical biochemistry in a cost-effective, rigorous fashion, exponentially advancing our capacity to investigate nature while hastening the advent of personalized medicine.


Mass Spectrometry Data processing Lipidomics Biostatistics Personalized medicine Precision medicine Metabolic flux analysis

Editors and affiliations

  • Angelo D'Alessandro
    • 1
  1. 1.Department of Biochemistry and Molecular GeneticsUniversity of Colorado DenverAuroraUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9235-5
  • Online ISBN 978-1-4939-9236-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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