Mass Spectrometry of Proteins

Methods and Protocols

  • Caroline A.  Evans
  • Phillip C. Wright
  • Josselin Noirel

Part of the Methods in Molecular Biology book series (MIMB, volume 1977)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. New Biological Insights from Technological Breakthroughs

    1. Front Matter
      Pages 1-1
    2. Qifeng Lin, Hwee Tong Tan, Maxey C. M. Chung
      Pages 3-15
    3. Joseph Longworth, Gunnar Dittmar
      Pages 25-34
    4. Peder J. Lund, Yekaterina Kori, Xiaolu Zhao, Simone Sidoli, Zuo-Fei Yuan, Benjamin A. Garcia
      Pages 43-70
    5. Paola Antinori, Théo Michelot, Pierre Lescuyer, Markus Müller, Adelina E. Acosta-Martin
      Pages 99-113
    6. David A. Russo, Narciso Couto, Andrew P. Beckerman, Jagroop Pandhal
      Pages 145-155
  3. Dealing with Proteomics Data in a Big Data Era

    1. Front Matter
      Pages 157-157
    2. Tomasz Burzykowski, Jürgen Claesen, Dirk Valkenborg
      Pages 181-197
    3. Dana Pascovici, Xiaomin Song, Jemma Wu, Thiri Zaw, Mark Molloy
      Pages 199-215
    4. Andrew F. Jarnuczak, Tobias Ternent, Juan Antonio Vizcaíno
      Pages 217-235
    5. Lars Malmström
      Pages 237-248
    6. Paul A. Stewart, Brent M. Kuenzi, Subina Mehta, Praveen Kumar, James E. Johnson, Pratik Jagtap et al.
      Pages 249-261
  4. Back Matter
    Pages 263-264

About this book


New insights into modern medicine and systems biology are enabled by innovative protocols and advanced technologies in mass spectrometry-based proteomics. This volume details new pipelines, workflows, and ways to process data that allow for new frontiers in proteomics to be pushed forward. With applications to biomarker discovery, interactions between proteins, between biological systems, dynamics of post-translational modifications among others, new protocols have been developed and iteratively refined to probe the endless complexity of the proteome in ever greater details. This volume deals with methods for data dependent and data independent mass spectrometry analyses. Valuable, first-hand information is provided from designing experiments, sample preparation and analysis, exploitation of public datasets and carrying out reproducible data pipelines, using modern computational tools such as Galaxy or Jupyter. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Mass Spectrometry of Proteins: Methods and Protocols aims to ensure successful results in the further study of this vital field. 


iTRAQ TMT di methyl labelling QConCat quantitative proteomics

Editors and affiliations

  • Caroline A.  Evans
    • 1
  • Phillip C. Wright
    • 2
  • Josselin Noirel
    • 3
  1. 1.Department of Chemical and Biological EngineeringUniversity of SheffieldSheffieldUK
  2. 2.Faculty of Science, Agriculture & EngineeringNewcastle UniversityNewcastle upon TyneUK
  3. 3.Laboratoire GBCM (EA4627), Conservatoire National des Arts et MétiersHESAM UniversitéParisFrance

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9231-7
  • Online ISBN 978-1-4939-9232-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
Industry Sectors
Consumer Packaged Goods