Cell Culture Techniques

  • Michael Aschner
  • Lucio Costa

Part of the Neuromethods book series (NM, volume 145)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Emma H. Neal, Yajuan Shi, Ethan S. Lippmann
    Pages 1-15
  3. Anke M. Tukker, Fiona M. J. Wijnolts, Aart de Groot, Richard W. Wubbolts, Remco H. S. Westerink
    Pages 17-35
  4. M. Diana Neely, Aaron B. Bowman
    Pages 37-49
  5. Lindsay T. Michalovicz, James P. O’Callaghan
    Pages 51-67
  6. S. Mancino, M. M. Serafini, Barbara Viviani
    Pages 69-85
  7. Monica Rodriguez-Silva, Kristen T. Ashourian, Anthony D. Smith, Jeremy W. Chambers
    Pages 87-118
  8. David P. Gavin, Xiaolu Zhang, Marina Guizzetti
    Pages 119-139
  9. Laura Nimtz, Jördis Klose, Stefan Masjosthusmann, Marta Barenys, Ellen Fritsche
    Pages 141-168
  10. Keturah G. Kiper, Jennifer L. Freeman
    Pages 169-193
  11. Gabriela Aguilera-Portillo, Aline Colonnello-Montero, Marisol Maya-López, Edgar Rangel-López, Abel Santamaría
    Pages 195-207
  12. Yukun Yuan, William D. Atchison
    Pages 209-231
  13. Zhen He, John Panos, James Raymick, Tetyana Konak, Li Cui, Diane B. Miller et al.
    Pages 233-243
  14. Cherish A. Taylor, Somshuvra Mukhopadhyay
    Pages 245-253
  15. Back Matter
    Pages 301-302

About this book


This volume discusses the requirements, advantages, and limitations of studying cell culture. The chapters in this book cover topics such as in vitro blood-brain barrier functional assays in human iPSC-based models; neuron-glia interactions examines with in vitro co-culture; epigenetic changes in cultures neurons and astrocytes; rat brain slices; brain punching technique; and using microRNA for in vitro neurotoxicity testing and related disorders. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.
Authoritative and cutting-edge, Cell Culture Techniques, Second Edition is a valuable resource for students and experiences researchers who are interested in learning more and making risk decisions in this evolving field.


Neurotoxicity in vitro iPSCs GCCP Neural Stem Cells

Editors and affiliations

  • Michael Aschner
    • 1
  • Lucio Costa
    • 2
  1. 1.Department of Molecular PharmacologyAlbert Einstein College of MedicineBronxUSA
  2. 2.Department of Environment/STE 100University of WashingtonSeattleUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9227-0
  • Online ISBN 978-1-4939-9228-7
  • Series Print ISSN 0893-2336
  • Series Online ISSN 1940-6045
  • Buy this book on publisher's site
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