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Computational Stem Cell Biology

Methods and Protocols

  • Patrick Cahan
Book

Part of the Methods in Molecular Biology book series (MIMB, volume 1975)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Modeling

  3. Single Cell Approaches

    1. Front Matter
      Pages 191-191
    2. Edroaldo Lummertz da Rocha, Mohan Malleshaiah
      Pages 193-209
    3. Thalia E. Chan, Michael P. H. Stumpf, Ann C. Babtie
      Pages 211-238
  4. Metabolism

    1. Front Matter
      Pages 303-303
    2. Fangzhou Shen, Camden Cheek, Sriram Chandrasekaran
      Pages 305-320
    3. Zhen Sun, Jing Zhao, Hua Yu, Chenyang Zhang, Hu Li, Zhongda Zeng et al.
      Pages 321-330
  5. Assessing and Improving Cell Fate Engineering

    1. Front Matter
      Pages 331-331
    2. John F. Ouyang, Uma S. Kamaraj, Jose M. Polo, Julian Gough, Owen J. L. Rackham
      Pages 333-361
    3. Hussein M. Abdallah, Domitilla Del Vecchio
      Pages 363-405
    4. Emily K. W. Lo, Patrick Cahan
      Pages 427-454
  6. John F. Ouyang, Uma S. Kamaraj, Jose M. Polo, Julian Gough, Owen J. L. Rackham
    Pages C1-C1
  7. Back Matter
    Pages 455-456

About this book

Introduction

This volume details methods and protocols to further the study of stem cells within the computational stem cell biology (CSCB) field. Chapters are divided into four sections covering the theory and practice of modeling of stem cell behavior, analyzing single cell genome-scale measurements, reconstructing gene regulatory networks, and metabolomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Computational Stem Cell Biology: Methods and Protocols will be an invaluable guide to researchers as they explore stem cells from the perspective of computational biology.

Keywords

gene regulatory networks single-cell transcriptome data teratomas engineered cells fetal tissues

Editors and affiliations

  • Patrick Cahan
    • 1
  1. 1.Department of Biomedical EngineeringJohns Hopkins UniversityBaltimoreUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-9224-9
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9223-2
  • Online ISBN 978-1-4939-9224-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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