Methods and Protocols

  • Joachim Clos

Part of the Methods in Molecular Biology book series (MIMB, volume 1971)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Dan Zilberstein, Roni Nitzan Koren
    Pages 1-8
  3. Katrin Kuhls, Isabel Mauricio
    Pages 9-68
  4. Hideo Imamura, Jean-Claude Dujardin
    Pages 69-94
  5. Peter J. Myler, Jacqueline A. McDonald, Pedro J. Alcolea, Aakash Sur
    Pages 95-108
  6. Amelie J. Kraus, Raúl O. Cosentino
    Pages 109-122
  7. Joachim Clos, Dorothea Zander-Dinse
    Pages 123-140
  8. Jade-Eva Potvin, Philippe Leprohon, Elodie Gazanion, Mansi Sharma, Christopher Fernandez-Prada, Marc Ouellette
    Pages 141-167
  9. Henner Zirpel, Joachim Clos
    Pages 169-188
  10. Samuel M. Duncan, Elmarie Myburgh, Eliza V. Alves-Ferreira, Jeremy C. Mottram
    Pages 211-224
  11. Jeziel D. Damasceno, Luiz R. O. Tosi, Renato E. R. S. Santos, Jeremy C. Mottram
    Pages 225-235
  12. Nuno Santarém, Joana Tavares, Anabela Cordeiro-da-Silva
    Pages 265-277
  13. Carolina Borsoi Moraes, Laura Maria Alcântara
    Pages 279-288
  14. Joana Tavares, Nuno Santarém, Anabela Cordeiro-da-Silva
    Pages 289-301
  15. María Dolores Jiménez-Antón, Montserrat Grau, Ana Isabel Olías-Molero, José Mª Alunda
    Pages 303-314
  16. Christian Bogdan, Andrea Debus, Heidi Sebald, Baplu Rai, Johanna Schäfer, Stephanie Obermeyer et al.
    Pages 315-349
  17. Back Matter
    Pages 369-370

About this book


This volume explores the latest techniques used to study Leishmania and other trypanosomatidae. Chapters in this book cover a wide range of topics such as cultivation of axenic amastigote forms; phylogeny and comparative genomics; and in vitro and in vivo infection models that look at hosts ranging from cell lines to mammalian and arthropode hosts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Unique and cutting-edge, Leishmania: Methods and Protocols is a valuable guide for both established and novice researchers in the Leishmania field. This book will also be useful to researchers working with other protozoa of the Order Trypanosomatide. 


Neglected Tropical Diseases Trypanosomae phylogenetic RT qPCR RNA seq

Editors and affiliations

  • Joachim Clos
    • 1
  1. 1.Bernhard Nocht Institute for Tropical MedicineHamburgGermany

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9209-6
  • Online ISBN 978-1-4939-9210-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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