Streptococcus pneumoniae

Methods and Protocols

  • Federico Iovino

Part of the Methods in Molecular Biology book series (MIMB, volume 1968)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Cultivation of Streptococcus pneumoniae In Vitro

    1. Front Matter
      Pages 1-1
  3. Microscopy Techniques to Study the Biology of Streptococcus pneumoniae and Pneumococcal Interactions with the Host

  4. The Genetics of Streptococcus pneumoniae

    1. Front Matter
      Pages 61-61
    2. Isabelle Mortier-Barrière, Nathalie Campo, Mathieu A. Bergé, Marc Prudhomme, Patrice Polard
      Pages 63-78
    3. Rory A. Eutsey, Carol A. Woolford, Surya D. Aggarwal, Rolando A. Cuevas, N. Luisa Hiller
      Pages 79-88
  5. The Proteome and Proteomics of Streptococcus pneumoniae

    1. Front Matter
      Pages 99-99
    2. María Dolores Cima-Cabal, Fernando Vazquez, Juan R. de los Toyos, María del Mar García-Suárez
      Pages 101-111
    3. Jianwei Zhou, Lu Zhang, Huixia Chuan, Angela Sloan, Raymond Tsang, Keding Cheng
      Pages 113-121
    4. Fang Ou, Cushla McGoverin, Joni White, Simon Swift, Frédérique Vanholsbeeck
      Pages 123-134
  6. Streptococcus pneumoniae-Host Interactions: In Vitro and In Vivo Models

    1. Front Matter
      Pages 135-135
    2. Yashuan Chao, Caroline Bergenfelz, Anders P. Hakansson
      Pages 147-171
    3. Federico Iovino, Vicky Sender, Birgitta Henriques-Normark
      Pages 173-181
    4. Miguel Palomino-Segura, Santiago F. Gonzalez
      Pages 183-194
    5. Adam Sierakowiak, Birgitta Henriques-Normark, Federico Iovino
      Pages 195-202
  7. Public Health, Epidemiology, and Biostatistics

    1. Front Matter
      Pages 203-203
    2. Godwin Oligbu, Norman K. Fry, Shamez N. Ladhani
      Pages 205-213
    3. Godwin Oligbu, Norman K. Fry, Shamez N. Ladhani
      Pages 215-224
  8. Back Matter
    Pages 225-227

About this book


This volume looks at the newest methods, materials, equipment, and technologies developed to study the cell biology of the pneumococcus. The chapters, which cover a wide range of topics, are organized into six parts: Part one discusses the cultivation of Streptococcus pneumoniae in vitro; Part Two talks about the microscopy techniques used to study the biology of Streptococcus pneumoniae and pneumococcal interactions with the host; Parts Three and Four describe the genetics, proteome, and proteomics of Streptococcus pneumoniae; Part Five explains in vitro and in vivo models of Streptococcus pneumoniae-host interactions; and Part Six looks at the factors of public health, epidemiology, and biostatistics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Streptococcus pneumoniae: Methods and Protocols is a valuable resource for both novice and expert researchers who want to expand their studies into new areas and new systems in this continuously evolving field.


cell division Epidemiology blood-agar plates Electron microscopy Western blotting immunoblotting Mass spectrometry

Editors and affiliations

  • Federico Iovino
    • 1
  1. 1.Department of Microbiology, Tumor and Cell BiologyKarolinska Institutet, BioclinicumStockholmSweden

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9198-3
  • Online ISBN 978-1-4939-9199-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
Industry Sectors
Health & Hospitals
Public Health
Internal Medicine & Dermatology