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Microcalorimetry of Biological Molecules

Methods and Protocols

  • Eric Ennifar

Part of the Methods in Molecular Biology book series (MIMB, volume 1964)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Differential Scanning Calorimetry

    1. Front Matter
      Pages 1-1
    2. Katherine Bowers, Natalia Markova
      Pages 33-44
    3. Philipp O. Tsvetkov, François Devred
      Pages 45-57
  3. Isothermal Titration Calorimetry

    1. Front Matter
      Pages 59-59
    2. Vaida Paketurytė, Asta Zubrienė, John E. Ladbury, Daumantas Matulis
      Pages 61-74
    3. Christopher P. Jones, Grzegorz Piszczek, Adrian R. Ferré-D’Amaré
      Pages 75-87
    4. Emma Schenckbecher, Benoît Meyer, Eric Ennifar
      Pages 89-98
    5. Ryo Amano, Tomohisa Furukawa, Taiichi Sakamoto
      Pages 119-128
    6. Benoît Meyer, Cyrielle da Veiga, Philippe Dumas, Eric Ennifar
      Pages 129-140
    7. Philipp O. Tsvetkov, Romain La Rocca, Soazig Malesinski, François Devred
      Pages 151-165
  4. Advanced Data Processing and Kinetics

    1. Front Matter
      Pages 167-167
    2. Rafael Claveria-Gimeno, Sonia Vega, Olga Abian, Adrian Velazquez-Campoy
      Pages 185-213
    3. Eva Muñoz, Juan Sabín, Javier Rial, Daniel Pérez, Eric Ennifar, Philippe Dumas et al.
      Pages 225-239
    4. Barbara Zambelli
      Pages 251-266
  5. Back Matter
    Pages 267-268

About this book

Introduction

This volume provides methods on microcalorimetry approaches to investigate complex biological molecular systems. Chapters guide readers through Differential Scanning Calorimetry (DSC), Isothermal Titration Calorimetry (ITC), and advanced data processing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Microcalorimetry of Biological Molecules: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Keywords

thermodynamic parameters structure-based drug design enzyme kinetics conformational changes isothermal titration

Editors and affiliations

  • Eric Ennifar
    • 1
  1. 1.Institut de Biologie Moléculaire et CellulaireUniversité de Strasbourg, CNRSStrasbourgFrance

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-9179-2
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9178-5
  • Online ISBN 978-1-4939-9179-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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