Ancient DNA

Methods and Protocols

  • Beth Shapiro
  • Axel Barlow
  • Peter D. Heintzman
  • Michael Hofreiter
  • Johanna L. A.  Paijmans
  • André E. R.  Soares

Part of the Methods in Molecular Biology book series (MIMB, volume 1963)

Table of contents

  1. Front Matter
    Pages i-x
  2. Tara L. Fulton, Beth Shapiro
    Pages 1-13
  3. Hannes Schroeder, Peter de Barros Damgaard, Morten E. Allentoft
    Pages 21-24
  4. Jesse Dabney, Matthias Meyer
    Pages 25-29
  5. Laura S. Epp, Heike H. Zimmermann, Kathleen R. Stoof-Leichsenring
    Pages 31-44
  6. Nathan Wales, Logan Kistler
    Pages 45-55
  7. Paula F. Campos, M. Thomas P. Gilbert
    Pages 57-63
  8. Kirstin Henneberger, Axel Barlow, Johanna L. A. Paijmans
    Pages 65-73
  9. Marie-Theres Gansauge, Matthias Meyer
    Pages 75-83
  10. Renata F. Martins, Marie-Louise Kampmann, Daniel W. Förster
    Pages 85-92
  11. Gloria González Fortes, Johanna L. A. Paijmans
    Pages 93-105
  12. Noah Snyder-Mackler, Tawni Voyles, Jenny Tung
    Pages 107-120
  13. André E. R. Soares
    Pages 121-128
  14. Johanna L. A. Paijmans, Gloria González Fortes, Daniel W. Förster
    Pages 129-139
  15. Ruth V. Nichols, Emily Curd, Peter D. Heintzman, Beth Shapiro
    Pages 149-161
  16. Gabriel Renaud, Mikkel Schubert, Susanna Sawyer, Ludovic Orlando
    Pages 163-194
  17. Back Matter
    Pages 215-216

About this book


This fully updated second edition explores protocols that address the most challenging aspects of experimental work in ancient DNA, such as preparing ancient samples for DNA extraction, the DNA extraction itself, and transforming extracted ancient DNA molecules for sequencing library preparation. The volume also examines the analysis of high-throughput sequencing data recovered from ancient specimens, which, because of the degraded nature of ancient DNA and common co-extraction of contaminant DNA, has challenges that are unique compared to data recovered from modern specimens.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Ancient DNA: Methods and Protocols, Second Edition aims to serve both experts and beginners by presenting protocols in a manner that makes them easily accessible for everyday use in the lab.


Fossil specimen Evolutionary research High-throughput sequencing Genomic library preparation Sequencing platforms DNA extraction

Editors and affiliations

  • Beth Shapiro
    • 1
  • Axel Barlow
    • 2
  • Peter D. Heintzman
    • 3
  • Michael Hofreiter
    • 4
  • Johanna L. A.  Paijmans
    • 5
  • André E. R.  Soares
    • 6
  1. 1.Department of Ecology and Evolutionary BiologyUniversity of California Santa CruzSanta CruzUSA
  2. 2.Institute for Biochemistry and BiologyUniversity of PotsdamPotsdamGermany
  3. 3.Tromsø University MuseumUiT—The Arctic University of NorwayTromsøNorway
  4. 4.Institute for Biochemistry and BiologyUniversity of PotsdamPotsdamGermany
  5. 5.Institute for Biochemistry and BiologyUniversity of PotsdamPotsdamGermany
  6. 6.Laboratório Nacional de Computação CientíficaPetrópolisBrazil

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9175-4
  • Online ISBN 978-1-4939-9176-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site