Proteomics for Biomarker Discovery

Methods and Protocols

  • Virginie Brun
  • Yohann Couté

Part of the Methods in Molecular Biology book series (MIMB, volume 1959)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Frank Klont, Peter Horvatovich, Natalia Govorukhina, Rainer Bischoff
    Pages 1-22
  3. Raghda Saad Zaghloul Taleb, Pacint Moez, Doreen Younan, Martin Eisenacher, Matthias Tenbusch, Barbara Sitek et al.
    Pages 51-64
  4. Armin Schniers, Yvonne Pasing, Terkel Hansen
    Pages 113-122
  5. Leslie Muller, Luc Fornecker, Sarah Cianferani, Christine Carapito
    Pages 123-127
  6. Alexandra Kraut, Mathilde Louwagie, Christophe Bruley, Christophe Masselon, Yohann Couté, Virginie Brun et al.
    Pages 129-150
  7. Jérôme Vialaret, Sylvain Lehmann, Christophe Hirtz
    Pages 163-172
  8. Didia Coelho Graça, Ralf Hartmer, Wolfgang Jabs, Alexander Scherl, Lorella Clerici, Kaveh Samii et al.
    Pages 173-183
  9. Carine Steiner, Pierre Lescuyer, Jean-Christophe Tille, Paul Cutler, Axel Ducret
    Pages 185-203
  10. Georgia Kontostathi, Manousos Makridakis, Vasiliki Bitsika, Nikolaos Tsolakos, Antonia Vlahou, Jerome Zoidakis
    Pages 205-223
  11. Samuel Wieczorek, Florence Combes, Hélène Borges, Thomas Burger
    Pages 225-246
  12. Xavier Robin
    Pages 261-273
  13. Lien Nguyen, Virginie Brun, Florence Combes, Valentin Loux, Yves Vandenbrouck
    Pages 275-289
  14. Back Matter
    Pages 291-292

About this book


This volume presents modern and enhanced methods that detail techniques to perform proteomics analyses dedicated to biomarker discovery for human health. Chapters guide readers through pre/post analytical factors, protocols for the preparation of extracellular vesicles and exosomes, and various analytical pipelines including Data Independent Acquisition (DIA), discovery, as well as targeted and top-down proteomics analysis workflows. Bioinformatics tools and workflows to select and evaluate candidate biomarkers or combinations of biomarkers are also presented. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Proteomics for Biomarker Discovery: Methods and Protocols aims to ensure successful results in the further study of this vital field.


Discovery proteomics analysis Biomarker candidates Precision medicine LC-MS/MS analysis Data science Pathogenic mechanisms

Editors and affiliations

  • Virginie Brun
    • 1
  • Yohann Couté
    • 2
  1. 1.Université Grenoble Alpes, CEA, Inserm, BGE U1038GrenobleFrance
  2. 2.Université Grenoble Alpes, CEA, Inserm, BGE U1038GrenobleFrance

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9163-1
  • Online ISBN 978-1-4939-9164-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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