T. cruzi Infection

Methods and Protocols

  • Karina Andrea Gómez
  • Carlos Andrés Buscaglia

Part of the Methods in Molecular Biology book series (MIMB, volume 1955)

Table of contents

  1. Front Matter
    Pages i-xvii
  2. Gonzalo Greif, Luisa Berná, Florencia Díaz-Viraqué, Carlos Robello
    Pages 35-45
  3. Noelia Lander, Miguel A. Chiurillo, Roberto Docampo
    Pages 61-76
  4. Carlos André Ornelas Ricart, Rayner Myr Lauterjung Queiroz, Agenor de Castro Moreira dos Santos Júnior, Sébastien Charneau
    Pages 77-87
  5. Izadora Volpato Rossi, Bruno Gavinho, Marcel Ivan Ramirez
    Pages 89-104
  6. María José Ferrer, Diana Patricia Wehrendt, Mariana Bonilla, Marcelo Alberto Comini, María Teresa Tellez-Iñón, Mariana Potenza
    Pages 105-118
  7. Virginia Balouz, Andrea C. Mesias, Camila Centeno Camean, Ivana Ducrey, Maite Mabel Lobo, Ignacio M. Durante et al.
    Pages 119-134
  8. Giannina Carlevaro, Andrés B. Lantos, Gaspar E. Cánepa, María de los Milagros Cámara, Martín Somoza, Carlos A. Buscaglia et al.
    Pages 135-146
  9. Martin C. Taylor, Amanda F. Francisco, Shiromani Jayawardhana, Gurdip Singh Mann, Alexander I. Ward, Francisco Olmo et al.
    Pages 147-163
  10. Matías Exequiel Rodríguez, Mariana Rizzi, Lucas Caeiro, Yamil Masip, Daniel O. Sánchez, Valeria Tekiel
    Pages 165-177
  11. Julien Santi-Rocca, Núria Gironès, Manuel Fresno
    Pages 187-202
  12. Juan Carlos Ramírez, Otacilio da Cruz Moreira
    Pages 215-225
  13. Otacilio da Cruz Moreira, Juan Carlos Ramirez
    Pages 227-238
  14. Gerd Wallukat, Katrin Wenzel, Ingolf Schimke
    Pages 247-261
  15. Elizabeth Ruiz-Lancheros, Makan Golizeh, Momar Ndao
    Pages 263-273
  16. Julio Alonso-Padilla, Dolors Tassies, Nuria Cortes-Serra, Joaquim Gascon, Joan-Carles Reverter, María-Jesús Pinazo
    Pages 275-286
  17. Nayara I. Medeiros, Juliana A. S. Gomes
    Pages 309-314
  18. Gonzalo R. Acevedo, Paula B. Alcaráz, Clemencia Pinilla, Karina A. Gómez
    Pages 315-337
  19. María Cecilia Albareda, María Ailén Natale, Gonzalo Leandro Cesar, Melisa Daiana Castro Eiro, María Gabriela Alvarez, Susana Adriana Laucella
    Pages 339-348
  20. Paola Lasso, Jose Mateus, John Mario González, Adriana Cuéllar, Concepción Puerta
    Pages 349-361
  21. Back Matter
    Pages 397-398

About this book


This volume provides detailed descriptions on cutting-edge experimental methods to assess the multiple and complex interactions between T. cruzi and its host. Chapters detail a series of methods and protocols, ranging from rather classical biochemical/genetic approaches to modern, powerful and high-throughput ‘-omics’ technologies, to tackle this issue, as well as novel techniques to improve treatment and clinical evaluation of Chagasic patients. Genome-wide mining strategies aimed at identifying potential parasite antigens and vaccine candidates and an overview on the challenges and milestones encountered during T. cruzi genome assembly are also included. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, T. cruzi Infection: Methods and Protocols aims to provide students, researchers, and clinicians who are interested in the study of Chagas disease an indispensable source of information.  


protozoan parasite T cell Exhaustion Cytometric Bead Array Chagas disease DC-PBMC

Editors and affiliations

  • Karina Andrea Gómez
    • 1
  • Carlos Andrés Buscaglia
    • 2
  1. 1.INGEBI-CONICETBuenos AiresArgentina
  2. 2.UNSAM-CONICETIIB-INTECHSan MartinArgentina

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9147-1
  • Online ISBN 978-1-4939-9148-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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