Target Identification and Validation in Drug Discovery

Methods and Protocols

  • Jürgen Moll
  • Sebastian Carotta

Part of the Methods in Molecular Biology book series (MIMB, volume 1953)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Identification of Novel Targets Using High Throughput Screening Assays

    1. Front Matter
      Pages 1-1
    2. Jennifer C. Volz, Nicole Schuller, Ulrich Elling
      Pages 3-21
    3. Konstantinos Drosopoulos, Spiros Linardopoulos
      Pages 33-42
    4. Martin Stöter, Antje Janosch, Rico Barsacchi, Marc Bickle
      Pages 43-60
  3. Drug Target Profiling and Validation

    1. Front Matter
      Pages 61-61
    2. Jean-Yves Trosset, Christian Cavé
      Pages 89-103
    3. Mahmoud Abdelatti, Peter Schofield, Daniel Christ
      Pages 121-136
  4. Three Dimensional Cell Culture Techniques Mimicking the Tumor Microenvironment In Vitro

  5. Animal Models for the Study of Gene Function In Vivo

    1. Front Matter
      Pages 181-181
    2. Dawei Chen, Xiaoyu An, Xuesong Ouyang, Jie Cai, Demin Zhou, Qi-Xiang Li
      Pages 183-211
    3. Alexander Klimke, Steffen Güttler, Petric Kuballa, Simone Janzen, Sonja Ortmann, Adriano Flora
      Pages 213-230
    4. Li-Chin Yao, Ken-Edwin Aryee, Mingshan Cheng, Pali Kaur, James G. Keck, Michael A. Brehm
      Pages 241-252
    5. Martina M. Sykora, Markus Reschke
      Pages 253-268
  6. Translational Methods to Validate Biomarkers

  7. Back Matter
    Pages 315-318

About this book


This second edition book explores breakthrough technologies in the field of drug target identification and validation. The volume emphasizes particularly revolutionary technologies, such as CRISPR-related screening, “big data,” and in silico approaches, as well as in vivo applications of CRISPR and best uses of animal models in drug development. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Fully updated and authoritative, Target Identification and Validation in Drug Discovery: Methods and Protocols, Second Edition is an ideal guide for molecular and cellular biologists, pharmacologists, pathologists, bioinformaticians, clinical researchers, or investigators, as well as experts in other fields that need a quick overview of these state-of-the-art technologies.


Drug development CRISPR technologies Gene function Animal models Pharmacodynamics Biomarkers Pharmaceutical properties

Editors and affiliations

  • Jürgen Moll
    • 1
  • Sebastian Carotta
    • 2
  1. 1.Pharmacology and Translational ResearchBoehringer Ingelheim RCV GmbH & Co KGViennaAustria
  2. 2.Department of Cancer Cell SignalingBoehringer Ingelheim RCV GmBH & Co KGViennaAustria

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9144-0
  • Online ISBN 978-1-4939-9145-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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