Adeno-Associated Virus Vectors

Design and Delivery

  • Michael J. Castle

Part of the Methods in Molecular Biology book series (MIMB, volume 1950)

Table of contents

  1. Front Matter
    Pages i-xii
  2. AAV Vector Design

    1. Front Matter
      Pages 1-1
    2. Florie Borel, Christian Mueller
      Pages 3-18
    3. Aman Patel, Junling Zhao, Dongsheng Duan, Yi Lai
      Pages 19-33
    4. Johanna Reul, Alexander Muik, Christian J. Buchholz
      Pages 35-50
    5. Julio Sanmiguel, Guangping Gao, Luk H. Vandenberghe
      Pages 51-83
    6. Emilie Lecomte, Adrien Leger, Magalie Penaud-Budloo, Eduard Ayuso
      Pages 85-106
    7. Jacqueline E. Hunter, Brittney L. Gurda, Sea Young Yoon, Michael J. Castle, John H. Wolfe
      Pages 107-122
  3. AAV Delivery to the Central Nervous System

    1. Front Matter
      Pages 141-141
    2. Dominic J. Gessler, Phillip W. L. Tai, Jia Li, Guangping Gao
      Pages 143-163
    3. Zeinab Noroozian, Kristiana Xhima, Yuexi Huang, Brian K. Kaspar, Sebastian Kügler, Kullervo Hynynen et al.
      Pages 177-197
    4. Cristina D. Peterson, Alexander G. J. Skorput, Kelley F. Kitto, George L. Wilcox, Lucy Vulchanova, Carolyn A. Fairbanks
      Pages 199-207
    5. Mariana Bravo-Hernández, Takahiro Tadokoro, Martin Marsala
      Pages 209-233
  4. AAV Delivery to Sensory Systems

    1. Front Matter
      Pages 235-235
    2. David C. Bloom, Zachary L. Watson, Donna M. Neumann
      Pages 237-247
    3. Paul D. Gamlin, John J. Alexander, Sanford L. Boye, C. Douglas Witherspoon, Shannon E. Boye
      Pages 249-262
    4. Jeffrey O’Callaghan, Matthew Campbell, Peter Humphries
      Pages 263-270
    5. Omar Akil, Lawrence Lustig
      Pages 271-282
    6. Akiyuki Taruno, Makiko Kashio
      Pages 299-307
  5. AAV Delivery to Other Tissues

    1. Front Matter
      Pages 309-309
    2. Estrella Lopez-Gordo, Erik Kohlbrenner, Michael G. Katz, Thomas Weber
      Pages 311-332
    3. Brett Palaschak, Roland W. Herzog, David M. Markusic
      Pages 333-360
    4. Laura P. van Lieshout, Jakob M. Domm, Sarah K. Wootton
      Pages 361-372
    5. Ping Guo, John Wiersch, Xiangwei Xiao, George Gittes
      Pages 373-387
    6. Wei Huang, Nicholas J. Queen, Lei Cao
      Pages 389-405
    7. Reshma Gore, Maureen S. Riedl, Kelley F. Kitto, Carolyn A. Fairbanks, Lucy Vulchanova
      Pages 407-415
  6. Back Matter
    Pages 417-426

About this book


This volume provides a complete and timely guide to the use of adeno-associated virus (AAV)  vectors for genetic manipulation of mammalian tissues. Beginning with methods for the design and characterization of AAV vectors, the book continues with protocols for AAV delivery to various components of the central nervous system, to a number of sensory systems, and to a broad range of other tissues. Novel techniques such as ultrasound-targeted delivery to the brain, subpial delivery to the spinal cord, and subILM delivery to the retina are accompanied by chapters that provide an overview and comparison of current methods for AAV delivery to tissues such as brain, heart, liver, and lung. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible step-by-step laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. 

Authoritative and comprehensive, Adeno-Associated Virus Vectors: Design and Delivery aims to enhance the utility of AAV vectors for targeted gene transfer to living animals and continue the ongoing development of novel AAV-based gene therapies for human disease.


Gene transfer Gene therapies AAV-based gene therapy Genetic manipulation CRISPR-mediated genome editing Central nervous system

Editors and affiliations

  • Michael J. Castle
    • 1
  1. 1.Department of NeurosciencesUniversity of California, San DiegoLa JollaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9138-9
  • Online ISBN 978-1-4939-9139-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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