© 2019

The Extracellular Matrix

Methods and Protocols

  • Davide Vigetti
  • Achilleas D. Theocharis
  • Includes cutting-edge techniques

  • Provides step-by-step detail essential for reproducible results

  • Contains key implementation advice from the experts


Part of the Methods in Molecular Biology book series (MIMB, volume 1952)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Dimitra Manou, Ilaria Caon, Panagiotis Bouris, Irene-Eva Triantaphyllidou, Cristina Giaroni, Alberto Passi et al.
    Pages 1-20
  3. Elena Caravà, Cristiana Marcozzi, Barbara Bartolini, Marcella Reguzzoni, Paola Moretto, Ilaria Caon et al.
    Pages 21-31
  4. Mario Raspanti, Marcella Reguzzoni, Petra Rita Basso, Marina Protasoni, Désirée Martini
    Pages 33-44
  5. Antonella Forlino, Francesca Tonelli, Roberta Besio
    Pages 45-53
  6. Mariana P. Stelling, Ananda A. de Bento, Philippe Caloba, Eduardo Vilanova, Mauro S. G. Pavão
    Pages 55-70
  7. Chiara Paganini, Rossella Costantini, Antonio Rossi
    Pages 71-80
  8. Daiana L. Vitale, Fiorella M. Spinelli, Laura Alaniz
    Pages 111-116
  9. Michela Bistoletti, Paola Moretto, Cristina Giaroni
    Pages 117-125
  10. Christos Velesiotis, Stella Vasileiou, Demitrios H. Vynios
    Pages 127-142
  11. Carolyn Chen, Aastha Kapoor, Renato V. Iozzo
    Pages 157-191
  12. Fatema Tuz Zahra, Efrossini Choleva, Md Sanaullah Sajib, Evangelia Papadimitriou, Constantinos M. Mikelis
    Pages 211-218
  13. Pinelopi Kastana, Fatema Tuz Zahra, Despoina Ntenekou, Stamatiki Katraki-Pavlou, Dimitris Beis, Michail S. Lionakis et al.
    Pages 219-232

About this book


This volume details established experimental protocols on the isolation, characterization, and detection of extracellular matrix (ECM) molecules as well as methods to study the activity and role of ECM components on various biological functions, the formation of exosomes, and tissue surrogates. Chapters display a variety of protocols ranging from biochemical, cell, and molecular biology assays to complex tissue imaging techniques and in vivo models to elucidate the role of the extracellular matrix. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, The Extracellular Matrix: Methods and Protocols aims to provide protocols and applications that can be used by both Ph.D. students and senior researchers to advance research in the field of extracellular matrix biology.


ECM molecules Cell-matrix interactions Drug delivery Matrix biology Exosomes Tissue surrogates

Editors and affiliations

  • Davide Vigetti
    • 1
  • Achilleas D. Theocharis
    • 2
  1. 1.Department of Medicine and SurgeryUniversity of InsubriaVareseItaly
  2. 2.Department of ChemistryUniversity of PatrasPatrasGreece

Bibliographic information

  • Book Title The Extracellular Matrix
  • Book Subtitle Methods and Protocols
  • Editors Davide Vigetti
    Achilleas D. Theocharis
  • Series Title Methods in Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-9132-7
  • eBook ISBN 978-1-4939-9133-4
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XII, 278
  • Number of Illustrations 22 b/w illustrations, 27 illustrations in colour
  • Topics Cell Biology
    Biochemistry, general
  • Buy this book on publisher's site
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