About this book
This book covers a wide range of state-of-the-art methodologies and detailed protocols currently used to study the actions that lipid-activated nuclear receptors and their co-regulators have in tissues and immune cell types considered classic metabolic “powerhouses”. This includes the liver, adipose tissue, and monocytes/macrophages present in these and other metabolic tissues. While the main focus is on the oxysterol receptor or Liver X Receptor (LXR), the majority of the methods described can be easily applied to multiple nuclear receptors, as well as to other tissues or cell types. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Lipid-Activated Nuclear Receptors: Methods and Protocols serves as an ideal guide for researchers pursuing the vital study of nuclear receptor biology and beyond.
Liver X receptors Transcriptional regulation Membrane lipids Immunity Oxysterol receptor
Editors and affiliations
- DOI https://doi.org/10.1007/978-1-4939-9130-3
- Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
- Publisher Name Humana Press, New York, NY
- eBook Packages Springer Protocols
- Print ISBN 978-1-4939-9129-7
- Online ISBN 978-1-4939-9130-3
- Series Print ISSN 1064-3745
- Series Online ISSN 1940-6029
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