Lipid-Activated Nuclear Receptors

Methods and Protocols

  • Matthew C. Gage
  • Inés Pineda-Torra

Part of the Methods in Molecular Biology book series (MIMB, volume 1951)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Lilia Magomedova, Carolyn L. Cummins
    Pages 1-14
  3. Jean-Baptiste Julla, Raphaelle Ballaire, Tina Ejlalmanesh, Jean-François Gautier, Nicolas Venteclef, Fawaz Alzaid
    Pages 33-48
  4. Ana Ramón-Vázquez, Juan Vladimir de la Rosa, Carlos Tabraue, Antonio Castrillo
    Pages 75-85
  5. Juan Vladimir de la Rosa, Ana Ramón-Vázquez, Carlos Tabraue, Antonio Castrillo
    Pages 99-109
  6. Sabrina Robichaud, Mireille Ouimet
    Pages 111-133
  7. Estibaliz Glaría, Jonathan Matalonga, Annabel F. Valledor
    Pages 135-141
  8. Ning Liang, Rongrong Fan, Saioa Goñi, Eckardt Treuter
    Pages 167-178
  9. Yasmine Sebti, Lise Ferri, Mathilde Zecchin, Justine Beauchamp, Denis Mogilenko, Bart Staels et al.
    Pages 189-207
  10. Kirsty E. Waddington, Inés Pineda-Torra, Elizabeth C. Jury
    Pages 209-216
  11. Back Matter
    Pages 217-220

About this book


This book covers a wide range of state-of-the-art methodologies and detailed protocols currently used to study the actions that lipid-activated nuclear receptors and their co-regulators have in tissues and immune cell types considered classic metabolic “powerhouses”. This includes the liver, adipose tissue, and monocytes/macrophages present in these and other metabolic tissues. While the main focus is on the oxysterol receptor or Liver X Receptor (LXR), the majority of the methods described can be easily applied to multiple nuclear receptors, as well as to other tissues or cell types. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Lipid-Activated Nuclear Receptors: Methods and Protocols serves as an ideal guide for researchers pursuing the vital study of nuclear receptor biology and beyond.


Liver X receptors Transcriptional regulation Membrane lipids Immunity Oxysterol receptor

Editors and affiliations

  • Matthew C. Gage
    • 1
  • Inés Pineda-Torra
    • 2
  1. 1.Division of MedicineCentre for Cardiometabolic Medicine, University College LondonLondonUK
  2. 2.Division of MedicineCentre for Cardiometabolic Medicine, University College LondonLondonUK

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9129-7
  • Online ISBN 978-1-4939-9130-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site