Methods and Protocols

  • Tim Bartels

Part of the Methods in Molecular Biology book series (MIMB, volume 1948)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Bin Zhang, Victoria Kehm, Ron Gathagan, Susan N. Leight, John Q. Trojanowski, Virginia M.-Y. Lee et al.
    Pages 45-57
  3. Rosalind F. Roberts, Nora Bengoa-Vergniory, Javier Alegre-Abarrategui
    Pages 69-76
  4. Thibaut Imberdis, Saranna Fanning, Andrew Newman, Nagendran Ramalingam, Ulf Dettmer
    Pages 77-91
  5. Mandy Koopman, Renée I. Seinstra, Ellen A. A. Nollen
    Pages 93-112
  6. Tara A. Eastwood, Daniel P. Mulvihill
    Pages 113-121
  7. Inês Caldeira Brás, Blagovesta Popova, Gerhard H. Braus, Tiago F. Outeiro
    Pages 145-156
  8. Eric S. Luth, Irina G. Stavrovskaya
    Pages 183-198
  9. Aron Szabo, George K. Tofaris
    Pages 199-208
  10. Ann Brinkmalm, Annika Öhrfelt, Payel Bhattacharjee, Henrik Zetterberg
    Pages 209-220
  11. Mahdi Muhammad Moosa, Josephine C. Ferreon, Allan Chris M. Ferreon
    Pages 221-233
  12. Ricardo Sanz, Patrick Ovando-Roche, Namrata D. Udeshi, Steven A. Carr, Chee Yeun Chung, Vikram Khurana
    Pages 235-246
  13. Julia Cattani, Theresa Braun, Malte Drescher
    Pages 247-260
  14. Alex E. Powers, Dushyant S. Patel
    Pages 261-269
  15. Akua A. Karikari, James B. Koprich, Chi Wang Ip
    Pages 271-286
  16. Back Matter
    Pages 287-289

About this book


This volume provides the reader with detailed protocols that describe a variety of in vitro and in vivo techniques that study reductionist systems and human samples. The methods covered in this book explore a broad selection of topics such as functional aspects of alpha-synuclein biology, its lipid-interactions and misfolding, and functional deficits in cells and mice. Chapters also cover topics such as kinetic measurements of endocytosis and exocytosis in cultured neurons; electrochemiluminescence-based detection; yeast-based screens to target alpha-synuclein toxicity; mass-spectrometry of alpha-synuclein in rat cortical neurons; and expression and purification of untagged a-synuclein. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Alpha-Synuclein: Methods and Protocols is a valuable reference guide that aids researchers working on topics related to alpha-synuclein and its various aspects.


endocytosis exocytosis amyloid formation protein-protein interaction rodent models toxicity

Editors and affiliations

  • Tim Bartels
    • 1
  1. 1.Dementia Research InstituteUniversity College LondonLondonUK

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9123-5
  • Online ISBN 978-1-4939-9124-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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