Table of contents
Probing Structural Properties of GPCRs
Tweaking Ligands for GPCR Studies
Bioluminescence and Fluorescence Resonance Transfer Energy Approaches
Exploring GPCR Signaling Properties
Visualizing Subcellular Compartmentalization of GPCRs
Investigating In Vivo Functions of GPCRs
About this book
This detailed volume assembles comprehensive protocols to assist with the study of structural, molecular, cell biological, and in vivo facets of GPCRs, and to enable the development of experimental tools for screening novel GPCR drugs. Sections explore the tweaking of ligands, bioluminescence and FRET approaches, specific GPCR signaling properties, as well as visualization of subcellular compartmentalization. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, G Protein-Coupled Receptor Signaling: Methods and Protocols serves as an ideal reference for life scientists working in a variety of research fields including molecular pharmacology, cell and developmental biology, brain behavior and physiology, drug development and screening.
Chapter 4 is available open access under a CC BY 4.0 license via link.springer.com.
Transmembrane protein families Molecular pharmacology Brain physiology Drug development Developmental biology GPCR drugs
Editors and affiliations
- DOI https://doi.org/10.1007/978-1-4939-9121-1
- Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
- Publisher Name Humana Press, New York, NY
- eBook Packages Springer Protocols
- Print ISBN 978-1-4939-9120-4
- Online ISBN 978-1-4939-9121-1
- Series Print ISSN 1064-3745
- Series Online ISSN 1940-6029
- Buy this book on publisher's site