Methods and Protocols

  • Irit Sagi
  • Nikolaos A. Afratis

Part of the Methods in Molecular Biology book series (MIMB, volume 1944)

Table of contents

  1. Front Matter
    Pages i-xi
  2. In Vitro Models for Collagen Characterization

    1. Front Matter
      Pages 1-1
    2. Olivier Bornert, Alexander Nyström
      Pages 3-15
    3. Ignacio Sallent, Héctor Capella-Monsonís, Dimitrios I. Zeugolis
      Pages 23-38
    4. Shasha Yao, Yifei Xu, Changyu Shao, Fabio Nudelman, Nico A. J. M. Sommerdijk, Ruikang Tang
      Pages 39-54
  3. Proteomics Analysis of Collagen

    1. Front Matter
      Pages 55-55
    2. Amar Joshi, Amna Zahoor, Alberto Buson
      Pages 79-93
    3. Ngoc-Duc Doan, Andrew S. DiChiara, Amanda M. Del Rosario, Richard P. Schiavoni, Matthew D. Shoulders
      Pages 95-114
    4. Elizabeta Madzharova, Fabio Sabino, Ulrich auf dem Keller
      Pages 115-126
  4. Imaging Approaches for Collagen

  5. Collagen in Health and Disease

  6. Back Matter
    Pages 253-254

About this book


This detailed volume compiles state-of-the-art protocols that will serve as recipes for scientists researching collagen, an abundant protein with great importance to health and disease, as well as in applications like food, cosmetics, pharmaceuticals, cosmetic surgery, artificial skin, and glue. Beginning with a section on in vitro models for the characterization of collagen formation, the book continues by highlighting large-scale analysis of collagen with mass spectrometry in order to elucidate the proteomics, degradomics, interactomes, and cross-linking of collagen, high resolution imaging approaches for collagen by the use of scanning electron microscopy and multiphoton imaging, as well as the role of collagen during physiological and pathological conditions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Collagen: Methods and Protocols is an ideal guide to high quality and repeatable protocols in this vital field of study.


Extracellular matrix Connective tissue Pathology Collagenous matrix Fibroblasts

Editors and affiliations

  • Irit Sagi
    • 1
  • Nikolaos A. Afratis
    • 2
  1. 1.Department of Biological RegulationWeizmann Institute of ScienceRehovotIsrael
  2. 2.Department of Biological RegulationWeizmann Institute of ScienceRehovotIsrael

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9094-8
  • Online ISBN 978-1-4939-9095-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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