Mouse Cell Culture

Methods and Protocols

  • Ivan Bertoncello

Part of the Methods in Molecular Biology book series (MIMB, volume 1940)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Practical Considerations

    1. Front Matter
      Pages 1-1
    2. Kristina Rydell-Törmänen, Jill R. Johnson
      Pages 3-22
    3. Ivan Bertoncello
      Pages 23-30
  3. Methods and Protocols

    1. Front Matter
      Pages 31-31
    2. Jacob M. Paynter, Joseph Chen, Xiaodong Liu, Christian M. Nefzger
      Pages 33-45
    3. Michael R. Larcombe, Jan Manent, Joseph Chen, Ketan Mishra, Xiaodong Liu, Christian M. Nefzger
      Pages 47-61
    4. Xiaodong Liu, Joseph Chen, Jaber Firas, Jacob M. Paynter, Christian M. Nefzger, Jose M. Polo
      Pages 63-76
    5. Tennille Sibbritt, Pierre Osteil, Xiaochen Fan, Jane Sun, Nazmus Salehin, Hilary Knowles et al.
      Pages 77-95
    6. Benjamin Cao, Songhui Li, Claire Pritchard, Brenda Williams, Susan K. Nilsson
      Pages 129-142
    7. Apoorva Mulay, Khondoker Akram, Lynne Bingle, Colin D. Bingle
      Pages 157-168
    8. Brigitte Müller
      Pages 181-191
    9. Matthew Ackers-Johnson, Roger S. Foo
      Pages 193-204
    10. Julie M. Sheridan, Jane E. Visvader
      Pages 217-229
    11. Deniz A. Bölükbas, Martina M. De Santis, Hani N. Alsafadi, Ali Doryab, Darcy E. Wagner
      Pages 275-295
    12. Xinhui Wu, Eline M. van Dijk, I. Sophie T. Bos, Loes E. M. Kistemaker, Reinoud Gosens
      Pages 297-311
  4. Back Matter
    Pages 313-315

About this book


This volume describes cell culture protocols for the maintenance, propagation, manipulation, and analysis of primary explanted cells from various mouse organ systems. After introductory chapters that examine the applicability of mouse models as a discovery tool and describe critical factors and variables that influence cell culture endpoints, protocol chapters explore cell culture methodologies for specific mouse cell types and lineages. Written in the highly successful Methods in Molecular Biology series format, these chapters include introductions to their respective topics, comprehensive lists of the essential materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Mouse Cell Culture: Methods and Protocols aims to ensure successful results utilizing these protocols in further studies, leading to the identification of key factors, genes, and cellular pathways regulating cell lineage commitment and differentiation, modeling disease, and identifying novel therapeutic targets.


Mouse organ systems Epithelial cells Organ development Cell lineage Stem cells

Editors and affiliations

  • Ivan Bertoncello
    • 1
  1. 1.Lung Health Research Centre, Department of Pharmacology and TherapeuticsUniversity of MelbourneMelbourneAustralia

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9085-6
  • Online ISBN 978-1-4939-9086-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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