Fragile-X Syndrome

Methods and Protocols

  • Dalit Ben-Yosef
  • Yoav Mayshar

Part of the Methods in Molecular Biology book series (MIMB, volume 1942)

Table of contents

  1. Front Matter
    Pages i-x
  2. FXS: Full Mutation

    1. Front Matter
      Pages 1-1
    2. Adi Reches
      Pages 3-10
    3. Xiaoqiang Cai, Mohammad Arif, Haolei Wan, Ruth Kornreich, Lisa J. Edelmann
      Pages 11-27
    4. Silvina Epsztejn-Litman, Rachel Eiges
      Pages 29-48
    5. Meng Li, Jack Faro Vander Stoep Hunt, Anita Bhattacharyya, Xinyu Zhao
      Pages 61-69
    6. Ulla-Kaisa Peteri, Maija L. Castrén
      Pages 71-78
    7. Jack Faro Vander Stoep Hunt, Meng Li, Xinyu Zhao, Anita Bhattacharyya
      Pages 79-88
    8. Liron Kuznitsov-Yanovsky, Yoav Mayshar, Dalit Ben-Yosef
      Pages 89-100
    9. Evelyn J. Sauter, Lisa K. Kutsche, Simon D. Klapper, Volker Busskamp
      Pages 101-121 Open Access
    10. Irena Vertkin, Dalit Ben-Yosef
      Pages 123-129
  3. FX Premutation

    1. Front Matter
      Pages 163-163
    2. Shai E. Elizur, Moran Friedman Gohas, Olga Dratviman-Storobinsky, Yoram Cohen
      Pages 165-171
    3. Marwa Zafarullah, Flora Tassone
      Pages 173-189
  4. Back Matter
    Pages 191-192

About this book


This volume discusses the latest technologies used to study all aspects of Fragile-X Syndrome (FXS). The chapters in this book cover topics such as monitoring for epigenetic modifications at the FMR1 locus; modeling FXS with human pluripotent stem cells, mouse neural progenitors; mouse versus human-based models for FXS pre-clinical research; and Fragile-X associated with Tremor/Ataxia Syndrome (FXTAS). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Fragile-X Syndrome: Methods and Protocols is a valuable tool to help scientists working towards one day developing a therapeutic solution to improve the symptoms of FXS.

Chapter "Induced Neurons for the Study of Neurodegenerative and Neurodevelopmental Disorders" is available open access under a Creative Commons Attribution 4.0 International License via


Ataxia Syndrome FXTAS drosophila c. elegans CRISPR

Editors and affiliations

  • Dalit Ben-Yosef
    • 1
  • Yoav Mayshar
    • 2
  1. 1.Wolfe PGD Stem Cell Lab, Racine IVF Unit at Lis Maternity HospitalTel Aviv Sourasky Medical Center, Tel Aviv UniversityTel AvivIsrael
  2. 2.Wolfe PGD Stem Cell Lab, Racine IVF Unit at Lis Maternity HospitalTel Aviv Sourasky Medical Center, Tel Aviv UniversityTel AvivIsrael

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9079-5
  • Online ISBN 978-1-4939-9080-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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