Table of contents

  1. Front Matter
    Pages i-xi
  2. Astrocyte Biology: Overviews and Perspectives

  3. Isolation of Astrocytes from Animal Models

    1. Front Matter
      Pages 35-35
    2. Yingbo He, Natalie Taylor, Anindya Bhattacharya
      Pages 37-47
  4. Astrocyte Morphology and Functions: Emerging Methods for Their Characterizations In Vivo, In Situ, and In Vitro

    1. Front Matter
      Pages 67-67
    2. Gaël Quesseveur, Aymeric Fouquier d’Hérouël, Keith K. Murai, David S. Bouvier
      Pages 85-95
    3. Marta Ramos, Nicholas Burdon Bechet, Roberta Battistella, Chiara Pavan, Anna L. R. Xavier, Maiken Nedergaard et al.
      Pages 97-104
    4. Olga Barca Mayo, Luca Berdondini, Davide De Pietri Tonelli
      Pages 131-154
    5. Niklas J. Gerkau, Karl W. Kafitz, Christine R. Rose
      Pages 187-202
    6. Victoria Adelheid Malik, Barbara Di Benedetto, Mira Jakovcevski
      Pages 219-229
  5. Astrocyte Pathologies in the Central Nervous System: Emerging Methods for Their Characterizations In Vivo, In Situ, and In Vitro

  6. Back Matter
    Pages 293-295

About this book


This detailed volume gathers together a broad variety of methods essential to the investigation of the biology of astrocytes and their multifaceted roles in both healthy and diseased brains. Beginning with some overviews of the subject, the book continues by covering techniques for the isolation of astrocytes from animal models, the investigation of astrocyte morphology and function, as well as for understanding astrocyte pathologies in the central nervous system. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Astrocytes: Methods and Protocols serves as an ideal guide for both experienced and beginner scientists working toward unraveling the novel, fascinating roles of these versatile cells.


Central nervous system (CNS) Neuronal networks Blood-brain barrier Neurogenesis Cellular circadian rhythm Astrocyte morphology Pathology

Editors and affiliations

  • Barbara Di Benedetto
    • 1
  1. 1.Department of Psychiatry and PsychotherapyUniversity of RegensburgRegensburgGermany

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9067-2
  • Online ISBN 978-1-4939-9068-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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