Methods and Protocols

  • Carmen Buchrieser
  • Hubert Hilbi

Part of the Methods in Molecular Biology book series (MIMB, volume 1921)

Table of contents

  1. Front Matter
    Pages i-xxvi
  2. Ecology and Physiology of Legionella

    1. Front Matter
      Pages 1-1
    2. Denise M. Burnside, Nicholas P. Cianciotto
      Pages 3-19
    3. Klaus Heuner, Mareike Kunze, Fan Chen, Wolfgang Eisenreich
      Pages 21-44
    4. Rosalie S. Braun, Nilmini Mendis, Laam Li, Sebastien P. Faucher
      Pages 45-53
    5. Ramon Hochstrasser, Hubert Hilbi
      Pages 79-89
  3. Legionella Genetics

    1. Front Matter
      Pages 91-91
    2. Nathalie Bailo, Hussein Kanaan, Elisabeth Kay, Xavier Charpentier, Patricia Doublet, Christophe Gilbert
      Pages 93-105
    3. Léo Hardy, Xavier Charpentier
      Pages 107-122
    4. Elizabeth Korevaar, Chen Ai Khoo, Hayley J. Newton
      Pages 145-157
  4. Cellular Microbiology of Legionella

    1. Front Matter
      Pages 159-159
    2. Monica Rolando, Carmen Buchrieser
      Pages 179-189
    3. Nicolas Personnic, Bianca Striednig, Hubert Hilbi
      Pages 191-204
    4. Ok-Ryul Song, Priscille Brodin, Carmen Buchrieser, Pedro Escoll
      Pages 205-220
    5. Bernhard Steiner, A. Leoni Swart, Hubert Hilbi
      Pages 221-238
  5. Biochemical Assays to Study Legionella Effectors and Enzymes

    1. Front Matter
      Pages 239-239
    2. Debnath Ghosal, Mohammed Kaplan, Yi-Wei Chang, Grant J. Jensen
      Pages 249-265
    3. Nadezhda Levanova, Irina Tabakova, Thomas Jank, Yury Belyi
      Pages 277-287
    4. Ernest C. So, Aurélie Mousnier, Gad Frankel, Gunnar N. Schroeder
      Pages 289-303
    5. Danielle P. A. Mascarenhas, Dario S. Zamboni
      Pages 305-319
  6. Immunity and Host Response Against Legionella

    1. Front Matter
      Pages 321-321
    2. Lina Scheithauer, Michael Steinert
      Pages 323-331
    3. Salvatore Bozzaro, Simona Buracco, Barbara Peracino, Ludwig Eichinger
      Pages 347-370
    4. Ann Karen C. Brassinga, Costi D. Sifri
      Pages 371-397
    5. Garrett Z. Ng, Anastasia Solomatina, Ian R. van Driel, Elizabeth L. Hartland
      Pages 399-417
  7. Metagenomics, Proteomics, and Host Microbiomes of Legionella

    1. Front Matter
      Pages 419-419
    2. Vincent Delafont, Yoann Perrin, Didier Bouchon, Laurent Moulin, Yann Héchard
      Pages 421-428
  8. Back Matter
    Pages 465-476

About this book


The methods described in the second edition on Legionella are for the study of distinct features of L. pneumophila.  Chapters guide readers through ecology and physiology of legionella, legionella genetics, cellular microbiology of legionella, biochemical assays to study legionella effectors and enzymes, immunity and host response against legionella, metagenomics, proteomics, and host microbiomes of legionella. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Legionella: Methods and Protocols, Second Edition aims to be useful for scientists studying Legionella,  and for a broader research community interested in the ecology, physiology, pathogenesis, immunity, genetics and evolution of other bacterial pathogens.


pneumophila Biofilm Amobea Imaging Flow Cytometry L. pneumophila Infection

Editors and affiliations

  • Carmen Buchrieser
    • 1
  • Hubert Hilbi
    • 2
  1. 1.Institut Pasteur, Biologie des Bactéries IntracellulairesParisFrance
  2. 2.Institute of Medical MicrobiologyUniversity of ZürichZürichSwitzerland

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9047-4
  • Online ISBN 978-1-4939-9048-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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