Plant Long Non-Coding RNAs

Methods and Protocols

  • Julia A. Chekanova
  • Hsiao-Lin V. Wang

Part of the Methods in Molecular Biology book series (MIMB, volume 1933)

Table of contents

  1. Front Matter
    Pages i-xvii
  2. Role of ncRNAs in Regulation of Gene Expression and Development in Plants

    1. Front Matter
      Pages 31-31
    2. Eundeok Kim, Yuqing Xiong, Byung-Ho Kang, Sibum Sung
      Pages 49-65
    3. Linqian Han, Lin Li, Gary J. Muehlbauer, John E. Fowler, Matthew M. S. Evans
      Pages 67-86
  3. Studying the Tissue and Cell-Type Specific lncRNAs

    1. Front Matter
      Pages 87-87
    2. Vibhav Gautam, Archita Singh, Sharmila Singh, Swati Verma, Ananda K. Sarkar
      Pages 89-98
    3. Edith Francoz, Philippe Ranocha, Christophe Dunand, Vincent Burlat
      Pages 99-130
    4. Trung Do, Zhipeng Qu, Iain Searle
      Pages 131-147
  4. Studying lncRNAs Associated with Response to Abiotic and Biotic Stresses in Plants

  5. Identification and Functional Analysis of lncRNAs

  6. Purification of lncRNAs and Ribonucleoprotein Complexes, RNA Secondary Structure, and RNA Modifications

  7. Databases of Plant lncRNAs and How to Use Them

    1. Front Matter
      Pages 395-395
    2. Andreu Paytuvi-Gallart, Walter Sanseverino, Riccardo Aiese Cigliano
      Pages 397-414
    3. Michał Wojciech Szcześniak, Oleksii Bryzghalov, Joanna Ciomborowska-Basheer, Izabela Makałowska
      Pages 415-429
    4. Bailing Zhou, Huiying Zhao, Jiafeng Yu, Chengang Guo, Xianghua Dou, Feng Song et al.
      Pages 431-437
  8. Long-Range Chromatin Interactions and Chromosome Conformation Capture

    1. Front Matter
      Pages 439-439
    2. Sudharsan Padmarasu, Axel Himmelbach, Martin Mascher, Nils Stein
      Pages 441-472
  9. Back Matter
    Pages 473-480

About this book


This volume focuses on various approaches to studying long non-coding RNAs (lncRNAs), including techniques for finding lncRNAs, localization, and observing their functions. The chapters in this book cover how to catalog lncRNAs in various plant species; determining subcellular localization; protein interactions; structures; and RNA modifications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and innovative, Plant Long Non-Coding RNAs: Methods and Protocols is a valuable resource that aids researchers in understanding the functions of lncRNAs in different plant species, and helps them explore currently uncharted facets of plant biology.


PATH library construction polyadenylated RNA Arabidopsis thaliana scaffold RNAs RNA-seq libraries

Editors and affiliations

  • Julia A. Chekanova
    • 1
  • Hsiao-Lin V. Wang
    • 1
    • 2
  1. 1.Guangxi Key Laboratory of Sugarcane BiologyGuangxi UniversityNanningChina
  2. 2.Department of BiologyEmory UniversityAtlantaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9044-3
  • Online ISBN 978-1-4939-9045-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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