Calcium-Binding Proteins of the EF-Hand Superfamily

From Basics to Medical Applications

  • Claus W. Heizmann

Part of the Methods in Molecular Biology book series (MIMB, volume 1929)

Table of contents

  1. Front Matter
    Pages i-xix
  2. Data Analysis and Modern Technologies to Study Calcium-Binding and Signaling in Cells

    1. Front Matter
      Pages 1-1
    2. Jacques Haiech, Marie-Claude Kilhoffer
      Pages 3-14
    3. Björn-Philipp Diercks, René Werner, Daniel Schetelig, Insa M. A. Wolf, Andreas H. Guse
      Pages 27-39
    4. Wei Zhang, Terunao Takahara, Takuya Achiha, Hideki Shibata, Masatoshi Maki
      Pages 95-109
    5. Mani Salarian, Shenghui Xue, Oluwatosin Y. Ibhagui, Jenny J. Yang
      Pages 111-125
    6. Masayuki Nara, Hisayuki Morii, Masaru Tanokura
      Pages 127-134
    7. Ravichandran Vignesh, Gopala Krishna Aradhyam
      Pages 135-154
  3. The Superfamily of Calcium-Binding Proteins Characterized by the EF-Hand Structural Motif

    1. Front Matter
      Pages 155-155
    2. Brian R. Thompson, Houda Cohen, Addeli Bez Batti Angulski, Joseph M. Metzger
      Pages 187-205
    3. Benjamin M. M. Grant, Christopher B. Marshall, Mitsuhiko Ikura
      Pages 207-221
    4. Hung Huy Nguyen, Peter Tompa, Kris Pauwels
      Pages 233-244
    5. Oleg Khassan, Katharine V. Jensen, Andrew G. Woodman, Hans J. Vogel, Hiroaki Ishida
      Pages 245-260
    6. Wenping Wu, Jimin Zheng, Zongchao Jia
      Pages 261-273
    7. Natalia Kozlyuk, Andrew J. Monteith, Velia Garcia, Steven M. Damo, Eric P. Skaar, Walter J. Chazin
      Pages 275-290
    8. Paul T. Wilder, Kristen M. Varney, David J. Weber
      Pages 291-310
    9. Bence Kiss, Péter Ecsédi, Márton Simon, László Nyitray
      Pages 325-338
    10. Noona Ambartsumian, Jörg Klingelhöfer, Mariam Grigorian
      Pages 339-365
    11. Kyohei Sakane, Fuminori Yamaguchi, Mitsumasa Tsuchiya, Rina Kondo, Naoki Kanayama, Masaki Magari et al.
      Pages 367-377
    12. Lisa S. Cunden, Elizabeth M. Nolan
      Pages 379-395
    13. Franziska Foertsch, Christian Melle
      Pages 447-460
    14. Markus Laube, Torsten Kniess, Christin Neuber, Cathleen Haase-Kohn, Jens Pietzsch
      Pages 461-485
    15. Wei Zhang, Rina Matsuo, Terunao Takahara, Hideki Shibata, Masatoshi Maki
      Pages 501-516
    16. Marco Mazzorana, Thomas Lykke-Møller Sørensen
      Pages 517-537
    17. Anand Kumar Sharma, Radhika Khandelwal, Yogendra Sharma
      Pages 551-566
    18. Uday Kiran, Michael R. Kreutz, Yogendra Sharma, Asima Chakraborty
      Pages 567-581
    19. Charlotte M. Thomas, David J. Timson
      Pages 615-641
  4. Biomarkers, Diagnostics and Laboratory Medicine

    1. Front Matter
      Pages 643-643
    2. Juan Martínez-Aguilar, Mark P. Molloy
      Pages 663-678
    3. Anna Lisa Frauchiger, Reinhard Dummer, Joanna Mangana
      Pages 691-700
    4. Diego Gazzolo, Francesca Pluchinotta, Giuseppe Lapergola, Simone Franchini
      Pages 701-728
    5. Mariagrazia Granata, Evangelia Skarmoutsou, Maria Clorinda Mazzarino, Fabio D’Amico
      Pages 729-738
    6. Gopalkrishna Sreejit, Sunil Kiran Nooti, Baskaran Athmanathan, Prabhakara Reddy Nagareddy
      Pages 739-754

About this book


This detailed volume explores protocols for studying the many facets of Ca2+-imaging, Ca2+-signaling, and Ca2+-binding along with background information on the principles and application of these techniques. The content of the book delves into 48 chapters including subjects such as data analysis and modern technologies to study calcium-binding and signaling in cells, the superfamily of calcium-binding proteins characterized by the EF-hand structural motif, as well as their use as diagnostic and prognostic biomarkers in Laboratory Medicine and novel therapeutic drug targets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and comprehensive, Calcium-Binding Proteins of the EF-Hand Superfamily: From Basics to Medical Applications presents state-of-the-art, lab-based methods and easy-to-follow protocols for daily use, making it interesting for basic and medical researchers, cell- and molecular biologists, clinicians, clinical chemists, and the diagnostic industry.


Ca2+-signaling Calcium-binding Therapeutic drug targets Personalized medicine Pharmacology

Editors and affiliations

  • Claus W. Heizmann
    • 1
  1. 1.Division of Clinical Chemistry and Biochemistry, Department of PediatricsUniversity of ZürichZürichSwitzerland

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9029-0
  • Online ISBN 978-1-4939-9030-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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