Kidney Organogenesis

Methods and Protocols

  • Seppo Vainio

Part of the Methods in Molecular Biology book series (MIMB, volume 1926)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Generation of Mosaic Embryonic Kidneys and Culture of the Kidney Tissues Ex Vivo

    1. Front Matter
      Pages 1-1
    2. Anneliis Ihermann-Hella, Satu Kuure
      Pages 23-30
    3. Filipa M. Lopes, Adrian S. Woolf
      Pages 31-38
    4. Aleksandra Rak-Raszewska
      Pages 39-51
  3. Embryonic Kidney Progenitor Cell and 3D Kidney Model Cell Like Based Cultures

    1. Front Matter
      Pages 53-53
    2. Andrew S. Potter, S. Steven Potter
      Pages 55-62
    3. Aaron C. Brown, Ashwani K. Gupta, Leif Oxburgh
      Pages 63-75
    4. Kai Zhang, Aki Manninen
      Pages 77-84
  4. Programming the Kidney Cell Fates in Human Pluripotent Cells

    1. Front Matter
      Pages 85-85
    2. Yasuhiro Yoshimura, Atsuhiro Taguchi, Ryuichi Nishinakamura
      Pages 87-102
    3. Krithika Hariharan, Petra Reinke, Andreas Kurtz
      Pages 103-115
    4. Shin-Ichi Mae, Makoto Ryosaka, Kenji Osafune
      Pages 117-123
  5. Protocols to Target the Functional Players in Kidney Ontogenesis

    1. Front Matter
      Pages 125-125
    2. Melanie L. Lawrence, Mona Elhendawi, Jamie A. Davies
      Pages 127-142
    3. Abhishek Sharma, Sandhanakrishnan Cattavarayane
      Pages 143-149
    4. Zhongwei Li, Toshikazu Araoka, Juan Carlos Izpisua Belmonte
      Pages 151-159
  6. 4D and 3D Ex Vivo Imaging of Embryonic Kidney Decellularization

    1. Front Matter
      Pages 161-161
    2. Ulla Saarela, Ilya Skovorodkin
      Pages 163-167
    3. Theresa Chow, Jennifer Whiteley, Ian M. Rogers
      Pages 169-184
    4. Renata Prunskaite-Hyyryläinen
      Pages 185-199
  7. Drosophila as a Model to Target Conserved Renal Ontogenesis Genes such as the Teashirt

    1. Front Matter
      Pages 201-201
    2. Shireen-A. Davies, Pablo Cabrero, Richard Marley, Guillermo Martinez Corrales, Saurav Ghimire, Anthony J. Dornan et al.
      Pages 203-221
    3. Laurent Fasano, Irene Sanchez-Martin, Xavier Caubit
      Pages 223-232
  8. Computational Approaches to Kidney Ontogenesis

    1. Front Matter
      Pages 233-233
    2. Pauli Tikka, Franz Schaefer
      Pages 235-246
  9. Back Matter
    Pages 247-248

About this book


This book details protocols on classical methods and very modern ex vivo mammalian developmental kidney model systems. Chapters focus on generation of mosaic 
embryonic kidneys, culture of the kidney tissues ex vivo, embryonic kidney progenitor cell, 3Dkidney model cell line based cultures, programming the kidney cell 
fates in human pluripotent cells, protocols to target the functional players in kidney ontogenesis, 4D and 3D ex vivo imaging, drosphila, and computational approaches to kidney ontogenesis. 
Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Kidney Organogenesis: Methods and Protocols aims to ensure successful results in the further study of this vital field.


nephrogenesis multipotent cells Drosophila tubulogenesis OMICS technologies

Editors and affiliations

  • Seppo Vainio
    • 1
  1. 1.Biocenter Oulu, Laboratory of Developmental BiologyOulu UniversityOuluFinland

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9020-7
  • Online ISBN 978-1-4939-9021-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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