Vertebrate Embryogenesis

Embryological, Cellular, and Genetic Methods

  • Francisco J. Pelegri

Part of the Methods in Molecular Biology book series (MIMB, volume 1920)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Thao Nguyen, Timothy J. Mitchison, Martin Wühr
    Pages 17-32
  3. Alexander A. Tokmakov, Ken-Ichi Sato
    Pages 41-57
  4. Laura S. Gammill, Bridget Jacques-Fricke, Julaine Roffers-Agarwal
    Pages 75-97
  5. Ana Paula Azambuja, Marcos Simoes-Costa
    Pages 99-110
  6. Karoline Holler, Jan Philipp Junker
    Pages 129-141
  7. Sonja Nowotschin, Vidur Garg, Anna Piliszek, Anna-Katerina Hadjantonakis
    Pages 163-182
  8. Edwina McGlinn, Miriam A. Holzman, Jennifer H. Mansfield
    Pages 183-218
  9. Matthew K. Vickaryous, Emily A. B. Gilbert
    Pages 219-246
  10. Jacqueline E. Moustakas-Verho, Rebecca McLennan, Jennifer Spengler, Paul M. Kulesa, Judith A. Cebra-Thomas
    Pages 247-263
  11. Amanda Butler, Dawn Owens, Mary Lou King, Tristan Aguero
    Pages 265-275
  12. KathyAnn L. Lee, Florence L. Marlow
    Pages 277-293
  13. Allison Jamieson-Lucy, Mary C. Mullins
    Pages 295-302
  14. Romain Gibeaux, Rebecca Heald
    Pages 303-315
  15. Young Hyun Park, Young Min Kim, Jae Yong Han
    Pages 317-326
  16. Taiju Saito, Rie Goto, Nicola Rivers, Etsuro Yamaha
    Pages 327-341
  17. Sukumal Prukudom, Kannika Siripattarapravat, William Poulos, Jose B. Cibelli
    Pages 353-375
  18. Dmitry Ershov, Nicolas Minc
    Pages 393-406
  19. Marcelo Couto, Charles Cates
    Pages 407-430
  20. Jacqueline E. Moustakas-Verho, Rebecca McLennan, Jennifer Spengler, Paul M. Kulesa, Judith A. Cebra-Thomas
    Pages C1-C1
  21. Back Matter
    Pages 431-434

About this book


This detailed volume explores animal embryogenesis in a way that aims to foster cross-model work and ideas by presenting methods that can be applied across laboratories and species boundaries. Improved protocols with updated advances in key traditional model systems are included, such as in amphibians, chicken, mouse, and zebrafish, as well as chapters on leopard gecko and the flexible-shelled slider turtle. Within these traditional model systems, new developments are presented, such as protocols for the analysis of cellular membranes and intracellular signals, light-controlled manipulation of function, and the analysis of transcriptomic and proteomic data in the context of the embryo. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and fully updated, Vertebrate Embryogenesis: Embryological, Cellular, and Genetic Methods, Second Edition serves as an invaluable aid to scientists, educators, and the advanced general audience and will act as an inspiration to further our understanding and appreciation of animal development.  

Chapter 10, "Optogenetic Control of Subcellular Protein Location and Signalling in Vertebrate Embryos," is available open access under a Creative Commons Attribution 4.0 International License via


Xenopus Zebrafish Balbiani body Mouse embryos CRISPR-Cas9 Model systems Non-traditional species

Editors and affiliations

  • Francisco J. Pelegri
    • 1
  1. 1.Laboratory of GeneticsUniversity of Wisconsin–MadisonMadisonUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9008-5
  • Online ISBN 978-1-4939-9009-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site