Bone Research Protocols

  • Aymen I.  Idris

Part of the Methods in Molecular Biology book series (MIMB, volume 1914)

Table of contents

  1. Front Matter
    Pages i-xv
  2. In Vitro Bone Cell Cultures

    1. Front Matter
      Pages 1-1
    2. Nadia Rucci, Alberta Zallone, Anna Teti
      Pages 3-19
    3. Inês P. Perpétuo, Lucie E. Bourne, Isabel R. Orriss
      Pages 21-38
    4. Jonathan H. Gooi, Ling Yeong Chia, Christina Vrahnas, Natalie A. Sims
      Pages 39-51
    5. Janos Kanczler, Rahul S. Tare, Patrick Stumpf, Timothy J. Noble, Cameron Black, Richard O. C. Oreffo
      Pages 53-70
    6. Silvia Marino, Ryan T. Bishop, Daniëlle de Ridder, Jesus Delgado-Calle, Michaela R. Reagan
      Pages 71-98
    7. Soraia P. Caetano-Silva, Astrid Novicky, Behzad Javaheri, Simon C. F. Rawlinson, Andrew A. Pitsillides
      Pages 99-128
  3. Biochemical and Molecular Analysis of Bone Cells

    1. Front Matter
      Pages 129-129
    2. Huilin Jin, Antonia Sophocleous, Asim Azfer, Stuart H. Ralston
      Pages 145-167
    3. Brice Moukengue, Jérôme Amiaud, Camille Jacques, Céline Charrier, Benjamin Ory, Francois Lamoureux
      Pages 169-196
  4. Ex Vivo Models

    1. Front Matter
      Pages 197-197
    2. Katherine A. Staines, Genevieve Brown, Colin Farquharson
      Pages 199-215
    3. Simon R. Goodyear, Richard M. Aspden
      Pages 241-257
  5. In Vivo Bone Models

    1. Front Matter
      Pages 259-259
    2. Antonia Sophocleous, Aymen I. Idris
      Pages 261-267
    3. Bettina Grötsch, Aline Bozec, Georg Schett
      Pages 269-280
    4. Antonia Sophocleous, Carmen Huesa
      Pages 281-293
    5. Sun Hee Park, Matthew Robert Eber, Yusuke Shiozawa
      Pages 295-308
    6. Claudia Tulotta, Arwin Groenewoud, B. Ewa Snaar-Jagalska, Penelope Ottewell
      Pages 309-330
    7. Camille Jacques, Nathalie Renema, Benjamin Ory, Carl R. Walkley, Agamemnon E. Grigoriadis, Dominique Heymann
      Pages 331-342
    8. Özge Uluçkan
      Pages 343-348
    9. Sam W. Z. Olechnowicz, Claire M. Edwards
      Pages 349-360
    10. Robert J. van ’t Hof
      Pages 361-368
    11. Behzad Javaheri, Nathalie Bravenboer, Astrid D. Bakker, Albert van der Veen, Roberto Lopes de Souza, Leanne Saxon et al.
      Pages 369-390
    12. Austen L. Thompson, Tally M. Largent-Milnes, Todd W. Vanderah
      Pages 391-407
  6. Microscopical and Imaging Techniques

    1. Front Matter
      Pages 409-409
    2. Reinhold G. Erben, Martin Glösmann
      Pages 411-435
    3. Anke J. Roelofs, Cosimo De Bari
      Pages 437-450
    4. Val Mann, Brendon Noble
      Pages 451-465
    5. Sarah L. Dallas, Patricia A. Veno, LeAnn M. Tiede-Lewis
      Pages 467-506
    6. Robert J. van ’t Hof, Enrico Dall’Ara
      Pages 507-531
    7. Jürg Andreas Gasser, Johannes Willnecker
      Pages 533-558
    8. Natalie C. Butterfield, John G. Logan, Julian Waung, Graham R. Williams, J. H. Duncan Bassett
      Pages 559-569
    9. Alan Boyde
      Pages 571-616
    10. Vincent Everts, Anneke Niehof, Wikky Tigchelaar-Gutter, Wouter Beertsen
      Pages 617-629
    11. Eleftherios P. Paschalis
      Pages 641-649
    12. Simon R. Goodyear, Richard M. Aspden
      Pages 651-659
  7. Back Matter
    Pages 661-670

About this book


This 3rd edition book provides are large number of laboratory protocols that are intended for basic and clinical bone researchers. Chapters are organized into five parts covering methods for isolation, ex vivo models of bone organ and cell co-culture system, biochemical procedures, animal models of bone remodeling, and rodent models on Rheumatoid arthritis and osteoarthritis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge Bone Research Protocols: Methods and Protocols, Third Edition aims to extensive list of protocols for anyone interested in bone research.


osteoclasts osteocytes stem cells Oestrogen deficiency Rheumatoid Arthritis

Editors and affiliations

  • Aymen I.  Idris
    • 1
  1. 1.Department of Oncology and MetabolismUniversity of SheffieldSheffieldUK

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8996-6
  • Online ISBN 978-1-4939-8997-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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