Plant Genome Editing with CRISPR Systems

Methods and Protocols

  • Yiping Qi

Part of the Methods in Molecular Biology book series (MIMB, volume 1917)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Review on Plant DNA Repair and Genome Editing

    1. Front Matter
      Pages 1-1
    2. Qiudeng Que, Zhongying Chen, Tim Kelliher, David Skibbe, Shujie Dong, Mary-Dell Chilton
      Pages 3-24
  3. CRISPR Design and Mutation Analysis

    1. Front Matter
      Pages 25-25
    2. Xianrong Xie, Xingliang Ma, Yao-Guang Liu
      Pages 33-43
  4. Multiplexed CRISPR-Cas9 Systems

    1. Front Matter
      Pages 45-45
    2. Riqing Li, Si Nian Char, Bing Yang
      Pages 47-61
    3. Xu Tang, Zhaohui Zhong, Qiurong Ren, Binglin Liu, Yong Zhang
      Pages 75-82
  5. CRISPR-Cas9 Editing in Monocots

    1. Front Matter
      Pages 95-95
    2. Weihang Gu, Dabing Zhang, Yiping Qi, Zheng Yuan
      Pages 97-107
    3. Jianping Zhou, Zhaohui Zhong, Hongqiao Chen, Qian Li, Xuelian Zheng, Yiping Qi et al.
      Pages 109-119
    4. Keunsub Lee, Huilan Zhu, Bing Yang, Kan Wang
      Pages 121-143
  6. CRISPR-Cas9 Editing in Dicots

    1. Front Matter
      Pages 145-145
    2. Tom Lawrenson, Penny Hundleby, Wendy Harwood
      Pages 155-170
    3. Nathan T. Reem, Joyce Van Eck
      Pages 171-182
    4. Satya Swathi Nadakuduti, Colby G. Starker, Daniel F. Voytas, C. Robin Buell, David S. Douches
      Pages 183-201
    5. Magdalena Klimek-Chodacka, Tomasz Oleszkiewicz, Rafal Baranski
      Pages 203-215
    6. Junqi Liu, Samatha Gunapati, Nicole T. Mihelich, Adrian O. Stec, Jean-Michel Michno, Robert M. Stupar
      Pages 217-234
    7. Hongge Jia, Xiuping Zou, Vladimir Orbovic, Nian Wang
      Pages 235-241
  7. CRISPR-Cas12a Editing Systems

    1. Front Matter
      Pages 243-243
    2. Yingxiao Zhang, Yong Zhang, Yiping Qi
      Pages 245-256
    3. Xiaojia Yin, Abhishek Anand, Paul Quick, Anindya Bandyopadhyay
      Pages 257-268
    4. Akira Endo, Seiichi Toki
      Pages 269-281
  8. Precise Gene Editing

    1. Front Matter
      Pages 283-283
    2. Jun Li, Xiangbing Meng, Jiayang Li, Caixia Gao
      Pages 285-296
    3. Zenpei Shimatani, Tohru Ariizumi, Ushio Fujikura, Akihiko Kondo, Hiroshi Ezura, Keiji Nishida
      Pages 297-307
  9. Non-Agrobacterium Based CRISPR Delivery Systems

    1. Front Matter
      Pages 309-309
    2. Ahmed Mahas, Zahir Ali, Manal Tashkandi, Magdy M. Mahfouz
      Pages 311-326
    3. Zhen Liang, Kunling Chen, Caixia Gao
      Pages 327-335
    4. Jongjin Park, Sunmee Choi, Slki Park, Jiyoung Yoon, Aiden Y. Park, Sunghwa Choe
      Pages 337-354
    5. Scott C. Lenaghan, C. Neal Stewart Jr.
      Pages 355-363
  10. Back Matter
    Pages 365-367

About this book


This volume provides readers with wide-ranging coverage of CRISPR systems and their applications in various plant species. The chapters in this book discuss topics such as plant DNA repair and genome editing; analysis of CRISPR-induced mutations; multiplexed CRISPR/Cas9 systems; CRISPR-Cas12a (Cpf1) editing systems; and non-agrobacterium based CRISPR delivery systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and thorough, Plant Genome Editing with CRISPR Systems: Methods and Protocols is a valuable resource for any researcher interested in learning about and using CRISPR systems in plants.


CRISPR DeCoding Sanger sequencing data Arabidopsis Cas9 genome Gene replacement

Editors and affiliations

  • Yiping Qi
    • 1
  1. 1.Department of Plant Science and Landscape ArchitectureUniversity of Maryland College ParkCollege ParkUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8990-4
  • Online ISBN 978-1-4939-8991-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
Industry Sectors
Consumer Packaged Goods