© 2019


Methods and Protocols

  • Jeannette S. Messer

Part of the Methods in Molecular Biology book series (MIMB, volume 1915)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Production and Purification of Calpains

    1. Front Matter
      Pages 1-1
    2. Christian-Scott E. McCartney, Peter L. Davies
      Pages 13-27
  3. Determination of Calpain Localization, Expression, and Activity

    1. Front Matter
      Pages 29-29
    2. Ashim Kumar Biswas, Samarth Tandon
      Pages 31-38
    3. Christian-Scott E. McCartney, Peter L. Davies
      Pages 39-55
    4. William Lostal, Daniel Stockholm, Isabelle Richard
      Pages 57-66
    5. Anna Charalambous, Ioanna Antoniades, Neophytos Christodoulou, Sara Zanardelli, Paris A. Skourides
      Pages 67-79
    6. Jean-Marie Exbrayat, Elara N. Moudilou, Claire Brun
      Pages 81-92
    7. Maira Cardoso, Danielle Oliveira, Helena Araujo
      Pages 93-101
  4. Identification of Calpain Substrates and Calpain-Activated Protein Functions

    1. Front Matter
      Pages 109-109
    2. Yong-Xian Fan, Xiaoyong Pan, Yang Zhang, Hong-Bin Shen
      Pages 111-120
    3. David A. duVerle, Hiroshi Mamitsuka
      Pages 121-147
    4. Maria del Carmen Lafita-Navarro, Maralice Conacci-Sorrell
      Pages 149-160
  5. Interrogation of Calpastatin, The Endogenous Calpain Inhibitor

    1. Front Matter
      Pages 161-161
    2. Roberta De Tullio, Monica Averna
      Pages 163-185
    3. Roberta De Tullio, Monica Averna
      Pages 187-194
    4. Monica Averna, Roberta De Tullio
      Pages 195-206

About this book


This volume brings together a plethora of protocols and experimental methods used by scientists to study calpains, their inhibitors, and their substrates. It also explores bioinformatic approaches to calpain substrate identification. The chapters in this book are divided into five parts and cover topics such as production and purification of calpains; determination of calpain localization, expression, and activity; identification of calpain-activated protein function; interrogation of calpastatin; and manipulation of calpain expression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Calpain: Methods and Protocols is a valuable resource for researchers and scientists who want to learn more about this developing field, and get inspired to make new discoveries that will aid in diagnosing and treating calpain-related diseases.


proteases enzymes Leukocytes inhibitors bioinformatic

Editors and affiliations

  • Jeannette S. Messer
    • 1
  1. 1.Department of Inflammation and ImmunityLerner Research Institute, Cleveland ClinicClevelandUSA

Bibliographic information

  • Book Title Calpain
  • Book Subtitle Methods and Protocols
  • Editors Jeannette S. Messer
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Springer, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-8987-4
  • eBook ISBN 978-1-4939-8988-1
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XII, 276
  • Number of Illustrations 29 b/w illustrations, 37 illustrations in colour
  • Topics Protein Science
  • Buy this book on publisher's site
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