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Co-Immunoprecipitation Methods for Brain Tissue

  • Yuji Odagaki
  • Dasiel O. Borroto-Escuela

Part of the Neuromethods book series (NM, volume 144)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Kirill Shumilov, Alejandra Valderrama-Carvajal, María García-Bonilla, Alicia Rivera
    Pages 109-122
  3. Wilber Romero-Fernandez, Maria Garcia-Barcelo, Yunis Perez-Betancourt
    Pages 137-145
  4. Michael Di Palma, Manuel Narvaez, Mariana Pita-Rodríguez, Chiara Contri, Martina Zannoni, Riccardo Cuppini et al.
    Pages 157-164
  5. Back Matter
    Pages 165-166

About this book

Introduction

This volume covers various aspects of co-immunoprecipitation (co-IP) methods and its relevant use in studying protein-protein interactions (PPIs) in health and diseases of the Central Nervous System. The chapters in this book discuss topics such as using co-IP to detect G protein-coupled receptors (GPCR), receptor tyrosine kinases (RTK) and ion channels heteroreceptor complexes in brain tissue; the histoblot technique; interaction strength between synaptic proteins using COS-7; and co-IP analysis of the protein-protein interactions in the neurons of Polymita. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Cutting-edge and thorough, Co-Immunoprecipitation Methods for Brain Tissue is a valuable resource for any researcher interesting in learning more about this developing field.

Keywords

PPI assay multiprotein complexes protein-protein interactions antibody lysate

Editors and affiliations

  • Yuji Odagaki
    • 1
  • Dasiel O. Borroto-Escuela
    • 2
  1. 1.Department of PsychiatrySaitama Medical UniversitySaitamaJapan
  2. 2.Department of NeuroscienceKarolinska InstitutetStockholmSweden

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-8985-0
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8984-3
  • Online ISBN 978-1-4939-8985-0
  • Series Print ISSN 0893-2336
  • Series Online ISSN 1940-6045
  • Buy this book on publisher's site
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