© 2019

Hepatitis C Virus Protocols

  • Mansun Law
  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts


Part of the Methods in Molecular Biology book series (MIMB, volume 1911)

Table of contents

  1. Front Matter
    Pages i-xix
  2. Review of Hepatitis C Virus and Bioinformatic Tools

    1. Front Matter
      Pages 1-1
    2. Yun Zhang, Christian Zmasek, Guangyu Sun, Christopher N. Larsen, Richard H. Scheuermann
      Pages 47-69
  3. Methods for Virus Cloning, Culture, and Purification

    1. Front Matter
      Pages 71-71
    2. Takanobu Kato, Asako Murayama, Takaji Wakita
      Pages 73-83
    3. Barnabas King, Richard Urbanowicz, Alexander W. Tarr, Jonathan K. Ball, C. Patrick McClure
      Pages 93-104
    4. Cynthia de la Fuente, Maria Teresa Catanese
      Pages 105-119
  4. Methods for the Study of HCV Life Cycle

    1. Front Matter
      Pages 137-137
    2. Victoria Castro, Ginés Ávila-Pérez, Lidia Mingorance, Pablo Gastaminza
      Pages 157-168
    3. Nicholas C. Wu, Hangfei Qi
      Pages 183-190
    4. Solène Denolly, François-Loïc Cosset, Natalia Freitas
      Pages 219-234
    5. Luhua Qiao, Guangxiang (George) Luo
      Pages 235-246

About this book


This volume details the most updated concepts and experimental protocols developed by leading researchers in the field. Chapters guide readers through methods on bioinformatics tools, hepatitis c virus(HCV) cloning, culture, and purification, HCV life cycle, host immune responses, and small animal models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Hepatitis C Virus Protocols aims to ensure successful results in the further study of this vital field.


virology Hepacivirus Flaviviridae pegiviruses pseudotype HCV

Editors and affiliations

  • Mansun Law
    • 1
  1. 1.Department of Immunology and MicrobiologyThe Scripps Research InstituteLa JollaUSA

Bibliographic information

  • Book Title Hepatitis C Virus Protocols
  • Editors Mansun Law
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-8975-1
  • eBook ISBN 978-1-4939-8976-8
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XIX, 508
  • Number of Illustrations 28 b/w illustrations, 47 illustrations in colour
  • Topics Immunology
  • Buy this book on publisher's site
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