Human Monoclonal Antibodies

Methods and Protocols

  • Michael Steinitz

Part of the Methods in Molecular Biology book series (MIMB, volume 1904)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Ilaria Marrocco, Donatella Romaniello, Yosef Yarden
    Pages 11-51
  3. Mahboobeh Fereidan-Esfahani, Tarek Nayfeh, Arthur Warrington, Charles L. Howe, Moses Rodriguez
    Pages 53-81
  4. Jenna J. Guthmiller, Haley L. Dugan, Karlynn E. Neu, Linda Yu-Ling Lan, Patrick C. Wilson
    Pages 109-145
  5. Hiroyuki Kishi, Tatsuhiko Ozawa, Hiroshi Hamana, Eiji Kobayashi, Atsushi Muraguchi
    Pages 147-162
  6. Nicole Ulmer, Sebastian Vogg, Thomas Müller-Späth, Massimo Morbidelli
    Pages 163-188
  7. Anna-Jasmina Donaubauer, Paul F. Rühle, Ina Becker, Rainer Fietkau, Udo S. Gaipl, Benjamin Frey
    Pages 189-212
  8. Taichi Kuramochi, Tomoyuki Igawa, Hiroyuki Tsunoda, Kunihiro Hattori
    Pages 213-230
  9. Nicolas Aubrey, Philippe Billiald
    Pages 231-252
  10. Susana Magadán Mompó, África González-Fernández
    Pages 253-291
  11. Keichiro Mihara, Tetsumi Yoshida, Joyeeta Bhattacharyya
    Pages 299-306
  12. Koji Hashimoto, Kohei Kurosawa, Hidetaka Seo, Kunihiro Ohta
    Pages 307-317
  13. Marco Dal Ferro, Serena Rizzo, Emanuela Rizzo, Francesca Marano, Immacolata Luisi, Olga Tarasiuk et al.
    Pages 319-338
  14. Fortunato Ferrara, Maria Felicia Soluri, Daniele Sblattero
    Pages 339-352
  15. Viola Fühner, Philip Alexander Heine, Kilian Johannes Carl Zilkens, Doris Meier, Kristian Daniel Ralph Roth, Gustavo Marçal Schmidt Garcia Moreira et al.
    Pages 353-375
  16. Angela Chiew Wen Ch’ng, Azimah Ahmad, Zoltán Konthur, Theam Soon Lim
    Pages 377-400
  17. Ivan Babic, Santosh Kesari, Mark C. Glassy
    Pages 401-415
  18. Dana Litvak-Greenfeld, Lilach Vaks, Stav Dror, Limor Nahary, Itai Benhar
    Pages 431-454
  19. Racheli Birnboim-Perach, Yehudit Grinberg, Lilach Vaks, Limor Nahary, Itai Benhar
    Pages 455-480
  20. Back Matter
    Pages 481-483

About this book


This second edition volume expands on the previous edition with descriptions of recent developments in the field. The chapters in this book cover topics such as monoclonal antibodies for the treatment of melanoma; production and purification of human monoclonal antibodies; humanization and optimization of monoclonal antibodies; rapid chimerization of monoclonal antibodies; epitope mapping via phage display from single gene libraries; recombinant antibodies made by combining phage and yeast display selections; production of stabilized antibody fragments in the E. coli bacterial cytoplasm and transfected mammalian cells; and analysis of CAR T cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Unique and thorough, Human Monoclonal Antibodies: Methods and Protocols, Second Edition is a valuable tool for novice and expert researchers interested in learning more about this evolving field.


transgenic mice phage display Alzheimer’s disease melanoma T-cells

Editors and affiliations

  • Michael Steinitz
    • 1
  1. 1.Department of PathologyThe Hebrew University, Hadassah Medical SchoolJerusalemIsrael

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8957-7
  • Online ISBN 978-1-4939-8958-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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