Methods and Protocols, Volume IV

  • Martha R. J. Clokie
  • Andrew Kropinski
  • Rob Lavigne

Part of the Methods in Molecular Biology book series (MIMB, volume 1898)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Jimena Barrero-Canosa, Cristina Moraru
    Pages 1-26
  3. Daniel Brownell, John King, Brian Caliando, Lada Sycheva, Michael Koeris
    Pages 37-50
  4. Nika Janež, Saša Haberl Meglič, Karel Flisar, Damijan Miklavčič, Matjaž Peterka
    Pages 51-56
  5. Hany Anany, Luba Y. Brovko, Denis Arutyunov, Nilufar Poshtiban, Amit Singh, Upasana Singh et al.
    Pages 89-105
  6. Lorena Rodríguez-Rubio, David M. Donovan, Beatriz Martínez, Ana Rodríguez, Pilar García
    Pages 107-115
  7. Jeroen De Smet, Hanne Hendrix, An Van den Bossche
    Pages 117-136
  8. Diana Vilas Boas, Carina Almeida, Nuno Azevedo, Sanna Sillankorva, Joana Azeredo
    Pages 137-146
  9. Hanne Hendrix, Ines Staes, Abram Aertsen, Jeroen Wagemans
    Pages 147-162
  10. Fatima Kamal, Danielle L. Peters, Jaclyn G. McCutcheon, Gary B. Dunphy, Jonathan J. Dennis
    Pages 163-171
  11. Jumpei Uchiyama, Iyo Takemura-Uchiyama, Shigenobu Matsuzaki
    Pages 173-181
  12. Fatima Kamal, Alina Radziwon, Carly M. Davis, Jonathan J. Dennis
    Pages 191-198
  13. Angélina Trotereau, Catherine Schouler
    Pages 199-205
  14. Andrew Chibeu, Sampathkumar Balamurugan
    Pages 207-214
  15. Back Matter
    Pages 215-216

About this book


This book expands on the previous volumes with new chapters focusing on functional characterization of phage and their proteins, and on the development of phage therapy by outlining novel models. The chapters in this book cover molecular topics such as PhageFISH for monitoring phage infections at single cell level; the analysis of phage-host protein-protein interactions using Strep-tag® II purifications; and also application driven chapters including ‘duckweed (Lemna minor) and alfalfa (Medicago sativa) as bacterial infection model systems’. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Innovative and thorough, Bacteriophages: Methods and Protocols, Volume IV is a valuable resource for both established and novice phage scientists.


Phage visualization Phage engineering Phage-protein analysis Phage therapy Model systems

Editors and affiliations

  • Martha R. J. Clokie
    • 1
  • Andrew Kropinski
    • 2
  • Rob Lavigne
    • 3
  1. 1.Department of Genetics and Genome BiologyUniversity of LeicesterLeicesterUK
  2. 2.Departments of Food Science, and PathobiologyUniversity of GuelphGuelphCanada
  3. 3.Laboratory of Gene TechnologyKU LeuvenLeuvenBelgium

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8939-3
  • Online ISBN 978-1-4939-8940-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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