Cellular Senescence

Methods and Protocols

  • Marco Demaria

Part of the Methods in Molecular Biology book series (MIMB, volume 1896)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Coralie Cazin, Mathieu von Joest, Han Li
    Pages 1-10
  3. Alessandro Galbiati, Fabrizio d’Adda di Fagagna
    Pages 11-20
  4. Stella Victorelli, João F. Passos
    Pages 21-29
  5. A. Vasilishina, A. Kropotov, I. Spivak, A. Bernadotte
    Pages 39-44
  6. Nicolas Malaquin, Véronique Tu, Francis Rodier
    Pages 45-55
  7. Irene Fernández-Duran, Núria Tarrats, Priya Hari, Juan Carlos Acosta
    Pages 57-70
  8. Dong Eun Kim, Albert R. Davalos
    Pages 71-81
  9. Andrew J. Innes, Jesús Gil
    Pages 83-92
  10. Dorothy N. Y. Fan, Clemens A. Schmitt
    Pages 93-105
  11. Hilah Gal, Ziv Porat, Valery Krizhanovsky
    Pages 107-117
  12. Sophia V. Rizou, Konstantinos Evangelou, Vassilios Myrianthopoulos, Iordanis Mourouzis, Sophia Havaki, Aikaterini Athanasiou et al.
    Pages 119-138
  13. Christopher D. Wiley, Sonnet Davis, Arvind Ramanathan
    Pages 139-147
  14. Joon Tae Park, Young-Sam Lee, Sang Chul Park
    Pages 149-157
  15. Hilda van den Bos, Bjorn Bakker, Aaron Taudt, Victor Guryev, Maria Colomé-Tatché, Peter M. Lansdorp et al.
    Pages 159-190
  16. Matthew J. Yousefzadeh, Kendra I. Melos, Luise Angelini, Christin E. Burd, Paul D. Robbins, Laura J. Niedernhofer
    Pages 203-230
  17. Lei Zhang, Jing Zhao, Aditi Gurkar, Laura J. Niedernhofer, Paul D. Robbins
    Pages 231-250
  18. Sophia V. Rizou, Konstantinos Evangelou, Vassilios Myrianthopoulos, Iordanis Mourouzis, Sophia Havaki, Aikaterini Athanasiou et al.
    Pages C1-C2
  19. Alessandro Galbiati, Fabrizio d’Adda di Fagagna
    Pages C3-C3
  20. Back Matter
    Pages 251-253

About this book


This book describes current methods for the identification and characterization of the major hallmarks of senescent cells. Chapters focus on the high heterogeneity of the senescence phenotypes, and techniques to induce and identify specific senescence programs. Additional chapters describe cellular and mouse models in which is possible to study the complex cell and non-cell autonomous functions of senescent cells.  Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cellular Senescence: Methods and Protocols aims to ensure successful results in the further study of this vital field.


senescence phenotype Reactive Oxygen Species DNA damage Senescence-Associated Secretory Phenotype Oncogene-Induced Senescence

Editors and affiliations

  • Marco Demaria
    • 1
  1. 1.ERIBAUniversity Medical Center GroningenGroningenThe Netherlands

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8930-0
  • Online ISBN 978-1-4939-8931-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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